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Literature summary extracted from

  • Kneidinger, B.; Graninger, M.; Adam, G.; Puchberger, M.; Kosma, P.; Zayni, S.; Messner, P.
    Identification of two GDP-6-deoxy-D-lyxo-4-hexulose reductases synthesizing GDP-D-rhamnose in Aneurinibacillus thermoaerophilus L420-91T (2001), J. Biol. Chem., 276, 5577-5583.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.281 overexpression in Escherichia coli Aneurinibacillus thermoaerophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.281 38100
-
 x * 38100, SDS-PAGE Aneurinibacillus thermoaerophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.281 GDP-6-deoxy-D-mannose + NAD(P)+ Aneurinibacillus thermoaerophilus
-
 GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.281 Aneurinibacillus thermoaerophilus
-
-
-
1.1.1.281 Aneurinibacillus thermoaerophilus Q6T1X6 L420-91T
-
1.1.1.281 Aneurinibacillus thermoaerophilus L420-91T Q6T1X6 L420-91T
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.281 recombinant enzyme Aneurinibacillus thermoaerophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.281 GDP-6-deoxy-D-lyxo-4-hexulose + NADH + H+
-
 Aneurinibacillus thermoaerophilus GDP-alpha-D-rhamnose + NAD+
-
 ir
1.1.1.281 GDP-6-deoxy-D-lyxo-4-hexulose + NADH + H+
-
 Aneurinibacillus thermoaerophilus L420-91T GDP-alpha-D-rhamnose + NAD+
-
 ir
1.1.1.281 GDP-6-deoxy-D-lyxo-4-hexulose + NADPH + H+
-
 Aneurinibacillus thermoaerophilus GDP-alpha-D-rhamnose + NADP+
-
 ir
1.1.1.281 GDP-6-deoxy-D-lyxo-4-hexulose + NADPH + H+
-
 Aneurinibacillus thermoaerophilus L420-91T GDP-alpha-D-rhamnose + NADP+
-
 ir
1.1.1.281 GDP-6-deoxy-D-mannose + NAD(P)+
-
 Aneurinibacillus thermoaerophilus GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.281 ? x * 38100, SDS-PAGE Aneurinibacillus thermoaerophilus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.281 GDP-4-keto-6-deoxy-D-mannose reductase (ambiguous)
-
 Aneurinibacillus thermoaerophilus
1.1.1.281 GDP-6-deoxy-D-lyxo-4-hexulose reductase
-
 Aneurinibacillus thermoaerophilus
1.1.1.281 RMD
-
 Aneurinibacillus thermoaerophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.281 NAD+
-
 Aneurinibacillus thermoaerophilus
1.1.1.281 NADH
-
 Aneurinibacillus thermoaerophilus
1.1.1.281 NADP+
-
 Aneurinibacillus thermoaerophilus
1.1.1.281 NADPH
-
 Aneurinibacillus thermoaerophilus