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Literature summary extracted from

  • Willows, R.D.; Beale, S.I.
    Heterologous expression of the Rhodobacter capsulatus BchI, -D, and -H genes that encode magnesium chelatase subunits and characterization of the reconstituted enzyme (1998), J. Biol. Chem., 273, 34206-34213.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.6.1.1 expression in Escherichia coli Rhodobacter capsulatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.6.1.1 Urea 20% inhibition with 100 mM, 50% inhibition with 250 mM, 90% inhibition with 800 mM Rhodobacter capsulatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.6.1.1 0.00123
-
 protoporphyrin IX pH 8.0, 30ºC Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O Rhodobacter capsulatus
-
 ADP + phosphate + Mg-protoporphyrin IX + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.6.1.1 Rhodobacter capsulatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.6.1.1 cation exchange chromatography Rhodobacter capsulatus

Reaction

EC Number Reaction Comment Organism Reaction ID
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ subunits H, I and D from bchH, bchI and bchD genes combine to form the enzyme complex, the complex is active only when the three proteins are present Rhodobacter capsulatus
a
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ this is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway Rhodobacter capsulatus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O
-
 Rhodobacter capsulatus ADP + phosphate + Mg-protoporphyrin IX + H+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6.1.1 8
-
-
 Rhodobacter capsulatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.6.1.1 additional information
-
 inactive below pH 6.0 and above pH 10.5 Rhodobacter capsulatus