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Literature summary extracted from

  • Willows, R.D.; Lake, V.; Roberts, T.H.; Beale, S.I.
    Inactivation of Mg chelatase during transition from anaerobic to aerobic growth in Rhodobacter capsulatus (2003), J. Bacteriol., 185, 3249-3258.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.6.1.1 BchD, BchI and BchH proteins are expressed in Escherichia coli from the respective cloned Rhodobacter capsulatus genes Rhodobacter capsulatus
6.6.1.1 expression of bchD gene in Escherichia coli Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O Rhodobacter capsulatus
-
 ADP + phosphate + Mg-protoporphyrin IX + H+
-
 ?
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O Rhodobacter capsulatus SB1003
-
 ADP + phosphate + Mg-protoporphyrin IX + H+
-
 ?
6.6.1.1 additional information Rhodobacter capsulatus BchH is the rate-limiting component of Mg chelatase in cell extracts, and its slective inactivation during adaption to aerobic growth may account for the rapid inactivation of Mg chelatase in vivo when anaerobically growing cells are exposed to O2 in the light ?
-
 ?
6.6.1.1 additional information Rhodobacter capsulatus SB1003 BchH is the rate-limiting component of Mg chelatase in cell extracts, and its slective inactivation during adaption to aerobic growth may account for the rapid inactivation of Mg chelatase in vivo when anaerobically growing cells are exposed to O2 in the light ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.6.1.1 Rhodobacter capsulatus P26162
-
-
6.6.1.1 Rhodobacter capsulatus P26162 BchH-deficient strain ZY6
-
6.6.1.1 Rhodobacter capsulatus P26162 SG1001 strain
-
6.6.1.1 Rhodobacter capsulatus SB1003 P26162 SG1001 strain
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ complex three-subunit enzyme Rhodobacter capsulatus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O
-
 Rhodobacter capsulatus ADP + phosphate + Mg-protoporphyrin IX + H+
-
 ?
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O
-
 Rhodobacter capsulatus SB1003 ADP + phosphate + Mg-protoporphyrin IX + H+
-
 ?
6.6.1.1 additional information BchH is the rate-limiting component of Mg chelatase in cell extracts, and its slective inactivation during adaption to aerobic growth may account for the rapid inactivation of Mg chelatase in vivo when anaerobically growing cells are exposed to O2 in the light Rhodobacter capsulatus ?
-
 ?
6.6.1.1 additional information BchH is the rate-limiting component of Mg chelatase in cell extracts, and its slective inactivation during adaption to aerobic growth may account for the rapid inactivation of Mg chelatase in vivo when anaerobically growing cells are exposed to O2 in the light Rhodobacter capsulatus SB1003 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.6.1.1 30
-
 assay at Rhodobacter capsulatus