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Literature summary extracted from

  • Gorchein, A.
    Cell-free activity of magnesium chelatase in Rhodobacter spheroides and Rhodobacter capsulatus (1997), Biochem. Soc. Trans., 25, 82S.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.6.1.1 thiomerosal 60% inhibition with 0.022 mM, in the absence of DTT Cereibacter sphaeroides
6.6.1.1 thiomerosal 60% inhibition with 0.022 mM, in the absence of DTT Rhodobacter capsulatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.6.1.1 0.00089
-
 protoporphyrin IX pH 8.2, 20-22ºC, 192000 * g supernatant Cereibacter sphaeroides
6.6.1.1 0.00089
-
 protoporphyrin IX pH 8.2, 20-22ºC, 192000 * g supernatant Rhodobacter capsulatus
6.6.1.1 0.00283
-
 protoporphyrin IX pH 8.2, 20-22ºC, 20000 * g supernatant Cereibacter sphaeroides
6.6.1.1 0.00283
-
 protoporphyrin IX pH 8.2, 20-22ºC, 20000 * g supernatant Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O Rhodobacter capsulatus
-
 ADP + phosphate + Mg-protoporphyrin IX + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.6.1.1 Cereibacter sphaeroides
-
-
-
6.6.1.1 Rhodobacter capsulatus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ this is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway Cereibacter sphaeroides
a
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ this is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway Rhodobacter capsulatus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O
-
 Rhodobacter capsulatus ADP + phosphate + Mg-protoporphyrin IX + H+
-
 ?
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O optimum activity at 11.5 mM Mg2+ Cereibacter sphaeroides ADP + phosphate + Mg-protoporphyrin IX + H+
-
 ?
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O optimum activity at 11.5 mM Mg2+ Rhodobacter capsulatus ADP + phosphate + Mg-protoporphyrin IX + H+
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.6.1.1 20 22 assay at Cereibacter sphaeroides
6.6.1.1 20 22 assay at Rhodobacter capsulatus