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Literature summary extracted from

  • Nakayama, M.; Masuda, T.; Sato, N.; Yamagata, H.; Bowler, C.; Ohta, H.; Shioi, Y.; Takamiya, K.
    Cloning, subcellular localization and expression of CHL1, a subunit of magnesium-chelatase in soybean (1995), Biochem. Biophys. Res. Commun., 215, 422-428.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.6.1.1 chloroplast
-
 Glycine max 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.6.1.1 40000
-
 x * 40000, SDS-PAGE, I subunit Glycine max
6.6.1.1 46000
-
 x * 46000, calculation from sequence of cDNA, I subunit Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O Glycine max
-
 ADP + phosphate + Mg-protoporphyrin IX + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.6.1.1 Glycine max
-
 soybean
-
6.6.1.1 Glycine max
-
 var Resnik
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ complex three-subunit enzyme Glycine max
a
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ this is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway Glycine max
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.6.1.1 cotyledon
-
 Glycine max
-
6.6.1.1 seed
-
 Glycine max
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O
-
 Glycine max ADP + phosphate + Mg-protoporphyrin IX + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
6.6.1.1 ? x * 46000, calculation from sequence of cDNA, I subunit Glycine max
6.6.1.1 ? x * 40000, SDS-PAGE, I subunit Glycine max
6.6.1.1 More the enzyme has three subunits, ChlI, ChlH and ChlD Glycine max