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Literature summary extracted from

  • Walker, C.J.; Weinstein, J.D.
    In vitro assay of the chlorophyll biosynthetic enzyme Mg-chelatase: resolution of the activity into soluble and membrane-bound fractions (1991), Proc. Natl. Acad. Sci. USA, 88, 5789-5793.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
6.6.1.1 26% loss of activity in chloroplasts subjected to hypotonic lysis and freeze-thaw cycles Pisum sativum

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.6.1.1 N-ethylmaleimide potent, IC50 of 0.02 mM Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.6.1.1 chloroplast
-
 Pisum sativum 9507
-
6.6.1.1 chloroplast
-
 Cucumis sativus 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.6.1.1 Mg2+ required Pisum sativum
6.6.1.1 Mg2+ required Cucumis sativus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O Pisum sativum
-
 ADP + phosphate + Mg-protoporphyrin IX + H+
-
 ?
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O Cucumis sativus
-
 ADP + phosphate + Mg-protoporphyrin IX + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.6.1.1 Cucumis sativus
-
 Sumter var
-
6.6.1.1 Pisum sativum
-
 pea
-
6.6.1.1 Pisum sativum
-
 var Bolero
-
6.6.1.1 Pisum sativum
-
 var Spring
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ complex three-subunit enzyme Pisum sativum
a
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ complex three-subunit enzyme Cucumis sativus
a
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ this is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway Pisum sativum
a
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ this is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway Cucumis sativus
a
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ linear reaction for at least 60 min under standard incubation Pisum sativum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.6.1.1 cotyledon
-
 Cucumis sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.6.1.1 additional information
-
 3fold to 4fold higher values than in cucumber chloroplasts Pisum sativum
6.6.1.1 0.0000024
-
 pellet of broken fractionated chloroplasts Pisum sativum
6.6.1.1 0.0000026
-
 supernatant of broken fractionated chloroplasts Pisum sativum
6.6.1.1 0.00002
-
 broken unfractionated chloroplasts Pisum sativum
6.6.1.1 0.0001
-
 intact chloroplasts Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O
-
 Pisum sativum ADP + phosphate + Mg-protoporphyrin IX + H+
-
 ?
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O
-
 Cucumis sativus ADP + phosphate + Mg-protoporphyrin IX + H+
-
 ?
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O omission of any of the substrates results in complete loss of activity. Optimum concentration of Mg2+ is lower for intact than broken and reconstituted chloroplasts Pisum sativum ADP + phosphate + Mg-protoporphyrin IX + H+
-
 ?

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.6.1.1 0.02
-
 potent, IC50 of 0.02 mM Pisum sativum N-ethylmaleimide