Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hohn, T.M.; Plattner, R.D.
    Purification and characterization of the sesquiterpene cyclase aristolochene synthase from Penicillium roqueforti (1989), Arch. Biochem. Biophys., 272, 137-143.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.9 Mn2+ above 0.01 mM, activation below Penicillium roqueforti
4.2.3.9 additional information no effect: phosphate up to concentrations of 5.0 mM Penicillium roqueforti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.9 0.00055
-
 trans,trans-farnesyl diphosphate pH 7.5 Penicillium roqueforti

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.9 Mg2+ required, maximal activity at 3 mM Penicillium roqueforti
4.2.3.9 Mn2+ 0.01 mM, can partially replace Mg2+, inhibition above Penicillium roqueforti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.9 37000
-
 1 * 37000, SDS-PAGE Penicillium roqueforti
4.2.3.9 48000
-
 gel filtration Penicillium roqueforti

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.9 Penicillium roqueforti
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.9
-
 Penicillium roqueforti

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.9 0.07
-
-
 Penicillium roqueforti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.9 trans,trans-farnesyl diphosphate
-
 Penicillium roqueforti aristolochene + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.9 monomer 1 * 37000, SDS-PAGE Penicillium roqueforti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.9 6.3 7.5
-
 Penicillium roqueforti