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Literature summary extracted from

  • Viswanathan, V.K.; Green, J.M.; Nicholas, B.P.
    Kinetic characterization of 4-amino 4-deoxychorismate synthase from Escherichia coli (1995), J. Bacteriol., 177, 5918-5923.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.6.1.85 glutamine prevents loss of activity Escherichia coli
2.6.1.85 pABA
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.85 additional information not inhibited by ATP, AMP, dAMP, guanosine, GMP, dGMP, GTP, deoxyguanosine, dTMP, serine, glycine, tyrosine, methionine, p-hydroxybenzoate, PABA, sulfanilamide Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.85 chorismate + L-glutamine Escherichia coli
-
 4-amino-4-deoxychorismate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.85 Escherichia coli
-
-
-
4.1.3.38 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.85 streptomycin sulfate precipitation, ammonium sulfate fractionation, chromatography on DEAE-cellulose Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.85 chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate the enzyme is composed of two parts, PabA and PabB Escherichia coli
a
2.6.1.85 chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate in the absence of PabA and glutamine, PabB converts ammonia and chorismate into 4-amino-4-deoxychorismate (in the presence of Mg2+) Escherichia coli
a
2.6.1.85 chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate non linear production of PABA in the ADC synthase-ADC lyase-coupled reaction system, at different glutamine and chorismate concentrations, with limiting PabB Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.85 chorismate + L-glutamine
-
 Escherichia coli 4-amino-4-deoxychorismate + L-glutamate
-
 ?
4.1.3.38 4-amino-4-deoxychorismate
-
 Escherichia coli 4-aminobenzoate + pyruvate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.85 37
-
 inactivation. DTT, chorismate or PabA prevent inactivation Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.3.38 pyridoxal 5'-phosphate
-
 Escherichia coli