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Literature summary extracted from

  • Ye, Q.Z.; Liu, J.; Walsh, C.T.
    p-Aminobenzoate synthesis in Escherichia coli: purification and characterization of PabB as aminodeoxychorismate synthase and enzyme X as aminodeoxychorismate lyase (1990), Proc. Natl. Acad. Sci. USA, 87, 9391-9395.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.85 additional information not inhibited by sulfathiazole Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.6.1.85 Mg2+
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.38 25000
-
 2 * 25000, SDS-PAGE Escherichia coli
4.1.3.38 50000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.85 chorismate + L-glutamine Escherichia coli
-
 4-amino-4-deoxychorismate + L-glutamate
-
 r
4.1.3.38 4-amino-4-deoxychorismate Escherichia coli
-
 4-aminobenzoate + pyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.85 Escherichia coli
-
-
-
4.1.3.38 Escherichia coli
-
 SG-5 cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.85 protamine sulfate precipitation, ammonium sulfate fractionation, chromatography on Sephacryl S-200, chromatography on DEAE-Sephacel and chromatography on dye-agarose Escherichia coli
4.1.3.38 DEAE-Sephacel, phenyl-Sepharose column, Superose 6 gel filtration column, Mono Q, Superose 12 column, yield 400-800fold Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.6.1.85 -20º, Tris-HCl buffer, pH 7.4, 50% glycerol Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.85 chorismate + L-glutamine
-
 Escherichia coli 4-amino-4-deoxychorismate + L-glutamate
-
 r
4.1.3.38 4-amino-4-deoxychorismate
-
 Escherichia coli 4-aminobenzoate + pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.38 dimer 2 * 25000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.38 37
-
 assay at Escherichia coli