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Literature summary extracted from

  • Hwang, Y.W.; Miller, D.L.
    A mutation that alters the nucleotide specificity of elongation factor Tu, a GTP regulatory protein (1987), J. Biol. Chem., 262, 13081-13085.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.6.5.3 medicine polypeptide chain synthesis Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.5.3
-
 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.5.3
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.6.5.3 D138N mutant with decreased affinity for GDP and increased affinity for XDP Escherichia coli

General Stability

EC Number General Stability Organism
3.6.5.3 EF-Tu(138N) not functional in vivo Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.5.3 43000
-
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.5.3 GDP + H2O Escherichia coli
-
 ?
-
 ?
3.6.5.3 GDP + H2O Escherichia coli HW110
-
 ?
-
 ?
3.6.5.3 GTP + H2O Escherichia coli
-
 GDP + phosphate
-
 ?
3.6.5.3 GTP + H2O Escherichia coli HW110
-
 GDP + phosphate
-
 ?
3.6.5.3 ITP + H2O Escherichia coli
-
 IDP + phosphate
-
 ?
3.6.5.3 ITP + H2O Escherichia coli HW110
-
 IDP + phosphate
-
 ?
3.6.5.3 XDP + H2O Escherichia coli
-
 XMP + phosphate
-
 ?
3.6.5.3 XDP + H2O Escherichia coli HW110
-
 XMP + phosphate
-
 ?
3.6.5.3 XTP + H2O Escherichia coli
-
 XDP + phosphate
-
 ?
3.6.5.3 XTP + H2O Escherichia coli HW110
-
 XDP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.5.3 Escherichia coli
-
 and strains EF-Tu (wt) and EF-Tu (138N)
-
3.6.5.3 Escherichia coli
-
 elongations factor (EF)Tu
-
3.6.5.3 Escherichia coli HW110
-
 and strains EF-Tu (wt) and EF-Tu (138N)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.5.3
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.5.3 aurodox + H2O
-
 Escherichia coli ?
-
 ?
3.6.5.3 GDP + H2O
-
 Escherichia coli ?
-
 ?
3.6.5.3 GDP + H2O
-
 Escherichia coli HW110 ?
-
 ?
3.6.5.3 GTP + H2O
-
 Escherichia coli GDP + phosphate
-
 ?
3.6.5.3 GTP + H2O
-
 Escherichia coli HW110 GDP + phosphate
-
 ?
3.6.5.3 ITP + H2O
-
 Escherichia coli IDP + phosphate
-
 ?
3.6.5.3 ITP + H2O
-
 Escherichia coli HW110 IDP + phosphate
-
 ?
3.6.5.3 XDP + H2O
-
 Escherichia coli XMP + phosphate
-
 ?
3.6.5.3 XDP + H2O
-
 Escherichia coli HW110 XMP + phosphate
-
 ?
3.6.5.3 XTP + H2O
-
 Escherichia coli XDP + phosphate
-
 ?
3.6.5.3 XTP + H2O
-
 Escherichia coli HW110 XDP + phosphate
-
 ?