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Literature summary extracted from

  • Illenberger, D.; Schwald, F.; Pimmer, D.; Binder, W.; Maier, G.; Dietrich, A.; Gierschik, P.
    Stimulation of phospholipase C-beta2 by the Rho GTPases Cdc42Hs and Rac1 (1998), EMBO J., 17, 6241-6249.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.5.2 expression in Escherichia coli Bos taurus
3.6.5.2 expression in Sf9 cells Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
3.6.5.2 F37A Cdc42Hs, fails to stimulate phospholipase C beta2 protein Bos taurus
3.6.5.2 Y40C Cdc42Hs, fails to stimulate phospholipase C beta2 protein Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.5.2 23000
-
 Cdc42Hs, SDS-PAGE Bos taurus
3.6.5.2 23000
-
 complex of 1 * 23000 for Cdc42Hs + 1 * 26000 for regulatory protein LyGDL, SDS-PAGE, immunostaining Homo sapiens
3.6.5.2 26000
-
 complex of 1 * 23000 for Cdc42Hs + 1 * 26000 for regulatory protein LyGDL, SDS-PAGE, immunostaining Homo sapiens
3.6.5.2 26000
-
 complex of 1 * 23000 for Cdc42Hs + 1 * 26000 for regulatory protein LyGDL, SDS-PAGE, immunostaining Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.5.2 GTP + H2O Homo sapiens
-
 GDP + phosphate
-
 ir
3.6.5.2 GTP + H2O Bos taurus
-
 GDP + phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.6.5.2 Bos taurus
-
-
-
3.6.5.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.5.2 recombinant enzymes, affinity chromatography Homo sapiens
3.6.5.2 recombinant enzymes, affinity chromatography Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.5.2 neutrophil
-
 Homo sapiens
-
3.6.5.2 neutrophil Cdc42Hs, Rac1, members of Rho GTPase subfamily Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.5.2 GTP + H2O
-
 Homo sapiens GDP + phosphate
-
 ir
3.6.5.2 GTP + H2O
-
 Bos taurus GDP + phosphate
-
 ir
3.6.5.2 guanosine 5'-O-(3-thiotriphosphate) + H2O poorly hydrolyzable, GTP[S]-activated Rac1 and Cdc42, but not RhoA, stimulate activity of phospholipase C-beta2 protein in complex with Rho GDP dissociation inhibitor protein LyGDL Homo sapiens guanosine 5'-O-diphosphate + thiophosphate
-
 ?
3.6.5.2 guanosine 5'-O-(3-thiotriphosphate) + H2O poorly hydrolyzable, GTP[S]-activated Rac1 and Cdc42, but not RhoA, stimulate activity of phospholipase C-beta2 protein in complex with Rho GDP dissociation inhibitor protein LyGDL Bos taurus guanosine 5'-O-diphosphate + thiophosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.5.2 dimer complex of 1 * 23000 for Cdc42Hs + 1 * 26000 for regulatory protein LyGDL, SDS-PAGE, immunostaining Homo sapiens
3.6.5.2 dimer complex of 1 * 23000 for Cdc42Hs + 1 * 26000 for regulatory protein LyGDL, SDS-PAGE, immunostaining Bos taurus

Synonyms

EC Number Synonyms Comment Organism
3.6.5.2 Cdc42Hs Rho subfamily Homo sapiens
3.6.5.2 Rac1 Rho subfamily Homo sapiens
3.6.5.2 Rac1 Rho subfamily Bos taurus
3.6.5.2 RhoA Rho subfamily Homo sapiens
3.6.5.2 RhoA Rho subfamily Bos taurus