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Literature summary extracted from

  • Suetfeld, R.; Towers, G.H.N.
    5-(4-Acetoxy-1-butynyl)-2,2'-bithiophene:acetate esterase from Tagetes patula (1982), Phytochemistry, 21, 277-279.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.54 bovine serum albumin activity is affected considerably, 5.0 mg/ml prevents substrate polymerization Tagetes patula

General Stability

EC Number General Stability Organism
3.1.1.54 after 6 months of repeated thawing and freezing about 50% of the esterase activity disappears Tagetes patula

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.54 bovine serum albumin BSA forms a weak conjugate with the substrate which inhibits the enzyme competitively Tagetes patula
3.1.1.54 additional information ascorbic acid, KCN at 0.001-100 mM, cysteine, glutathione, dithiothreitol, 5,5'-dithiobis(2-nitrobenzoate), 4-chloromercuribenzoate at 0.001-1 mM are no inhibitors Tagetes patula

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.54 0.02
-
 5-(4-Acetoxybut-1-ynyl)-2,2'-bithiophene pH 8.0, 30°C Tagetes patula

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.54 67000
-
 gel filtration Tagetes patula

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.54 5-(4-acetoxybut-1-ynyl)-2,2'-bithiophene + H2O Tagetes patula enzyme involved in the transformation of an acetylenic compound 5-(4-hydroxy-but-1-ynyl)-2,2'-bithiophene + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.54 Tagetes patula
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.54 partial Tagetes patula

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.54 leaf
-
 Tagetes patula
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.54 0.0156
-
-
 Tagetes patula

Storage Stability

EC Number Storage Stability Organism
3.1.1.54 -20°C, purified enzyme can be stored for more than 3 months without a marked loss of activity Tagetes patula

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.54 5-(4-acetoxybut-1-ynyl)-2,2'-bithiophene + H2O
-
 Tagetes patula 5-(4-hydroxy-but-1-ynyl)-2,2'-bithiophene + acetate
-
 ?
3.1.1.54 5-(4-acetoxybut-1-ynyl)-2,2'-bithiophene + H2O enzyme involved in the transformation of an acetylenic compound Tagetes patula 5-(4-hydroxy-but-1-ynyl)-2,2'-bithiophene + acetate
-
 ?
3.1.1.54 additional information none of various acetate esters as well as other naturally occuring esters like chlorogenic acid or p-coumaroyl-CoA are hydrolysed by the enzyme Tagetes patula ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.54 90
-
 preparation heated for 5 min is inactive Tagetes patula

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.54 7.5
-
-
 Tagetes patula