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Literature summary extracted from

  • Hirao, T.; Takahashi, K.
    Purification and characterization of a calcium-activated neutral protease from monkey brain and its action on neuropeptides (1984), J. Biochem., 96, 775-784.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.53 antipain
-
 Macaca fuscata
3.4.22.53 E-64
-
 Macaca fuscata
3.4.22.53 leupeptin
-
 Macaca fuscata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.22.53 Ca2+ requires millimolar order calcium ions for activation Macaca fuscata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.53 20000
-
 1 * 74000 + 1 * 20000, SDS-PAGE Macaca fuscata
3.4.22.53 74000
-
 1 * 74000 + 1 * 20000, SDS-PAGE Macaca fuscata
3.4.22.53 110000
-
 gel filtration Macaca fuscata

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.53 Macaca fuscata
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.53 brain
-
 Macaca fuscata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.53 additional information
-
-
 Macaca fuscata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.53 alpha-neoendorphin + H2O cleavage of the 6Arg-7Lys bond Macaca fuscata ?
-
 ?
3.4.22.53 angiotensin + H2O clevage of the 4tyr-5Ile bond Macaca fuscata ?
-
 ?
3.4.22.53 beta-lipotropin(61-91) + H2O cleavage of the bonds: Thr76-Leu77, Lys84-Asn85 and Lys88-Lys89 Macaca fuscata ?
-
 ?
3.4.22.53 beta-neoendorphin + H2O cleavage of the 6Arg-7Lys bond Macaca fuscata ?
-
 ?
3.4.22.53 casein + H2O
-
 Macaca fuscata ?
-
 ?
3.4.22.53 dynorphin (1-13) + H2O cleavage of the 6Arg-7Arg bond Macaca fuscata ?
-
 ?
3.4.22.53 Leu-enkephalin + H2O cleaved only slightly at the 1Tyr-2Gly bond Macaca fuscata ?
-
 ?
3.4.22.53 Met-enkephalin + H2O cleaved only slightly at the 1Tyr-2Gly bond Macaca fuscata ?
-
 ?
3.4.22.53 neurotensin + H2O cleavage of the 3Tyr-4Glu bond and the 5Asn-6Lys bond Macaca fuscata ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.53 dimer 1 * 74000 + 1 * 20000, SDS-PAGE Macaca fuscata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.53 7.5 8
-
 Macaca fuscata