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Literature summary extracted from

  • Giffhorn, F.; Rode, H.; Kuhn, A.; Gottschalk, G.
    Citrate lyase deacetylase of Rhodopseudomonas gelatinosa. Isolation of the enzyme and studies on the inhibition by L-glutamate (1980), Eur. J. Biochem., 111, 461-471.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.2.16 loses activity upon repeated freezing and thawing Rubrivivax gelatinosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.16 alpha-glutamyl-glutamate 10 mM, 70% inhibition Rubrivivax gelatinosus
3.1.2.16 D-glutamate 10 mM, 54% inhibition Rubrivivax gelatinosus
3.1.2.16 gamma-glutamyl-glutamate 10 mM, 70% inhibition Rubrivivax gelatinosus
3.1.2.16 L-aspartate 10 mM, 60% inhibition Rubrivivax gelatinosus
3.1.2.16 L-glutamate linear mixed type of inhibition Rubrivivax gelatinosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.16 7300
-
 2 * 7300, SDS-PAGE Rubrivivax gelatinosus
3.1.2.16 14300
-
 gel filtration Rubrivivax gelatinosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.16 [citrate (pro-3S)-lyase](acetyl form) + H2O Rubrivivax gelatinosus regulates the activity of citrate layse by covalent modification [citrate (pro-3S)-lyase](thiol form) + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.16 no activity in Enterobacter aerogenes
-
-
-
3.1.2.16 no activity in Escherichia coli
-
-
-
3.1.2.16 no activity in Streptococcus diacetilactis
-
-
-
3.1.2.16 Rubrivivax gelatinosus
-
 DSM 149
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.16
-
 Rubrivivax gelatinosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.2.16 additional information
-
 specific activity 1000 U/mg Rubrivivax gelatinosus
3.1.2.16 additional information
-
 specific activity 15.00 U/mg Rubrivivax gelatinosus

Storage Stability

EC Number Storage Stability Organism
3.1.2.16 -20°C, partly purified enzyme retains full activity for at least 1 year Rubrivivax gelatinosus
3.1.2.16 -20°C, purified enzyme loses 20% of activity within 1 year during storage Rubrivivax gelatinosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.16 [citrate (pro-3S)-lyase](acetyl form) + H2O
-
 Rubrivivax gelatinosus [citrate (pro-3S)-lyase](thiol form) + acetate
-
 ?
3.1.2.16 [citrate (pro-3S)-lyase](acetyl form) + H2O regulates the activity of citrate layse by covalent modification Rubrivivax gelatinosus [citrate (pro-3S)-lyase](thiol form) + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.16 dimer 2 * 7300, SDS-PAGE Rubrivivax gelatinosus

Synonyms

EC Number Synonyms Comment Organism
3.1.2.16 acetyl-S-(acyl-carrier protein) enzyme thioester hydrolase (acetate)
-
 Rubrivivax gelatinosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.2.16 50
-
 half-life 20 min, effectively protected against heat inactivation by 100 mM L-glutamate, half-life 30 min Rubrivivax gelatinosus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.2.16 0.12
-
 L-glutamate pH 7.5, 30°C Rubrivivax gelatinosus