Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kuroda, H.; Miyadera, A.; Imura, A.; Suzuki, A.
    Partial purification, and some properties and reactivities of cetraxate benzyl ester hydrochloride-hydrolyzing enzyme (1989), Chem. Pharm. Bull., 37, 2929-2932.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.70 EDTA
-
 Aspergillus niger
3.1.1.70 eserine
-
 Aspergillus niger

Application

EC Number Application Comment Organism
3.1.1.70 medicine cetraxate hydrochloride is a widely used antiulcer agent industrially produced Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.70 Ca2+ 1.0 mM, 71% relative residual activity Aspergillus niger
3.1.1.70 Co2+ 1.0 mM, 68% relative residual activity Aspergillus niger
3.1.1.70 Cu2+ 1.0 mM, 72% relative residual activity Aspergillus niger
3.1.1.70 diisopropyl phosphofluoridate 9% relative residual activity Aspergillus niger
3.1.1.70 Fe2+ 1.0 mM, 81% relative residual activity Aspergillus niger
3.1.1.70 Hg2+ 9% relative residual activity Aspergillus niger
3.1.1.70 Mg2+ 1.0 mM, 64% relative residual activity Aspergillus niger
3.1.1.70 Mn2+ 1.0 mM, 79% relative residual activity Aspergillus niger
3.1.1.70 Ni2+ 1.0 mM, 67% relative residual activity Aspergillus niger
3.1.1.70 o-phenanthroline 80% relative residual activity Aspergillus niger
3.1.1.70 Zn2+ 1.0 mM, 70% relative residual activity Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.70 4
-
 benzyl cetraxate pH 4.5, 30°C Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.70 35000
-
 gel filtration Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.70 cetraxate benzyl ester + H2O Aspergillus niger
-
 cetraxate + benzyl alcohol
-
 ?
3.1.1.70 L-Phe benzyl ester + H2O Aspergillus niger
-
 benzyl alcohol + L-phenylalanine
-
 ?
3.1.1.70 L-Tyr benzyl ester + H2O Aspergillus niger
-
 benzyl alcohol + L-tyrosine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.70 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.70 partial Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.70 commercial preparation Sumizyme AC, Shin-Nippon Chemical Industries Co., Ltd., cellulase activity 2-5 unit/mg Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.70 additional information
-
 specific activity 791 units/mg Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.70 benzyl esters of substituted phenyl propanoates + H2O benzyl esters of aliphatic and aromatic carboxylic acids, benzyl esters of phenylalanine Phe-By and tyrosine Tyr-By are hydrolyzed Aspergillus niger benzyl alcohol + substituted phenyl propanoate
-
 ?
3.1.1.70 cetraxate benzyl ester + H2O
-
 Aspergillus niger cetraxate + benzyl alcohol
-
 ?
3.1.1.70 L-Phe benzyl ester + H2O
-
 Aspergillus niger benzyl alcohol + L-phenylalanine
-
 ?
3.1.1.70 L-Tyr benzyl ester + H2O
-
 Aspergillus niger benzyl alcohol + L-tyrosine
-
 ?
3.1.1.70 additional information enzyme shows no cellulase, protease or lipase activity, does not hydrolyze ester groups such as Tyr-Me, Tyr-tert-Bu, 1-Me, 1-iso-Pro and 1 Ph Aspergillus niger ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.70 monomer 1 * 35000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.70 40
-
 debenzylation Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.70 40
-
 stable at temperatures below Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.70 4.5
-
 for debenzylating substrate Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.70 4 7 stable in this range Aspergillus niger

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.70 Aspergillus niger isoelectric focusing, carrier ampholite, pH range from 3.5-10.0 at 200 V for 30 min and 700 V for 90 min
-
 5.3