EC Number | General Stability | Organism |
---|---|---|
3.1.1.57 | aged enzyme preparation is fully reactivated by preincubation at 24°C for 10 min with an excess amount of dithiothreitol, cysteine or GSH | Pseudomonas straminea |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.57 | Co(NO3)2 | inhibits hydrolysis reaction by 59.9% | Pseudomonas straminea | |
3.1.1.57 | Cu(NO3)2 | inhibits synthesis reaction | Pseudomonas straminea | |
3.1.1.57 | HgCl2 | - |
Pseudomonas straminea | |
3.1.1.57 | Mn(NO3)2 | inhibits hydrolysis reaction by 62.1% | Pseudomonas straminea | |
3.1.1.57 | additional information | monoiodoacetate had essentially no effect, Mg(NO3)2, Ni(NO3)2, Ca(NO3)2, Cr(NO3)3 and Fe(NO3)3 shows essentially no effect, KCN and various metal chelating reagents including EDTA, tiron, 1,10-phenanthroline, 2,2'-bipyridine, diethyldithiocarbonate and 8-hydroxyquinoline shows no significant effect | Pseudomonas straminea | |
3.1.1.57 | N-ethylmaleimide | - |
Pseudomonas straminea | |
3.1.1.57 | p-chloromercuribenzoate | - |
Pseudomonas straminea | |
3.1.1.57 | Zn(NO3)2 | inhibits hydrolysis reaction | Pseudomonas straminea |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.1.57 | 0.026 | - |
4-oxalmesaconate | pH 6.5, 24°C | Pseudomonas straminea | |
3.1.1.57 | 0.087 | - |
2-Pyrone-4,6-dicarboxylate | pH 8.5, 24°C | Pseudomonas straminea |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.57 | 31000 | - |
gel filtration | Pseudomonas straminea |
3.1.1.57 | 33000 | - |
1 * 33000, SDS-PAGE | Pseudomonas straminea |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.57 | 2-pyrone-4,6-dicarboxylate + H2O | Pseudomonas straminea | highly specific, various other lactones does not serve as substrates | 4-carboxy-2-hydroxyhexa-2,4-dienedioate | product isomerizes to 4-oxalmesaconate | r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.57 | Pseudomonas straminea | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.57 | - |
Pseudomonas straminea |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.57 | 247 | - |
purification of hydrolase activity | Pseudomonas straminea |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.1.57 | -20°C, 30% of the activity is lost on storage for 1 year | Pseudomonas straminea |
3.1.1.57 | -20°C, purified enzyme can be stored for at least 2 months without appreciable loss in activity | Pseudomonas straminea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.57 | 2-pyrone-4,6-dicarboxylate + H2O | highly specific | Pseudomonas straminea | 4-carboxy-2-hydroxyhexa-2,4-dienedioate | product isomerizes to 4-oxalmesaconate | r | |
3.1.1.57 | 2-pyrone-4,6-dicarboxylate + H2O | highly specific, various other lactones does not serve as substrates | Pseudomonas straminea | 4-carboxy-2-hydroxyhexa-2,4-dienedioate | product isomerizes to 4-oxalmesaconate | r | |
3.1.1.57 | additional information | no hydrolysis of gamma-butyrolactone, gamma-valerolactone, epsilon-caprolactone, gamma-n-decalactone, D-glucorono-gamma-lactone, mevalonolactone, 2-pyrone-6-carboxylate, coumalic acid, isodehydracetic acid, 4-hydroxy-6-methyl-2-pyrone and 4-methoxy-6-methyl-2-H-pyran-2-one | Pseudomonas straminea | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.57 | monomer | 1 * 33000, SDS-PAGE | Pseudomonas straminea |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.57 | 2-pyrone-4,6-dicarboxylate hydrolase | - |
Pseudomonas straminea |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.57 | 6 | 7.5 | 2-pyrone-4,6-dicarboxylate synthesis | Pseudomonas straminea |
3.1.1.57 | 8.5 | - |
2-pyrone-4,6-dicarboxylate hydrolysis | Pseudomonas straminea |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.1.57 | Pseudomonas straminea | isolelectric focusing at 6°C, LKB 8101 column pH 3.5-10.0 | - |
5.49 |