EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.8.3.9 | Tris-HCl | activation at 0.25 M, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate | Clostridium sp. |
EC Number | General Stability | Organism |
---|---|---|
2.8.3.9 | purified enzyme is very stable, considerably less stable in Tris-HCl than in phosphate buffer | Clostridium sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.8.3.9 | 0.0034 | - |
acetoacetyl-CoA | pH 7.0, 25°C, cosubstrate crotonate | Clostridium sp. | |
2.8.3.9 | 0.023 | - |
butyryl-CoA | pH 7.0, 25°C, cosubstrate crotonate | Clostridium sp. | |
2.8.3.9 | 0.034 | - |
butyryl-CoA | pH 7.0, 25°C, cosubstrate acetoacetate | Clostridium sp. | |
2.8.3.9 | 0.044 | - |
butyryl-CoA | pH 6.3, 25°C, cosubstrate acetoacetate | Clostridium sp. | |
2.8.3.9 | 0.054 | - |
butyryl-CoA | pH 8.1, 25°C, cosubstrate acetoacetate 1.0 mM, 20 mM MgCl2 | Clostridium sp. | |
2.8.3.9 | 0.073 | - |
butyryl-CoA | pH 8.1, 25°C, cosubstrate acetoacetate 2.0 mM, 20 mM MgCl2 | Clostridium sp. | |
2.8.3.9 | 0.083 | - |
butyryl-CoA | pH 8.1, 25°C, cosubstrate acetoacetate 3.0 mM, 20 mM MgCl2 | Clostridium sp. | |
2.8.3.9 | 0.135 | - |
acetyl-CoA | pH 7.0, 25°C, cosubstrate acetoacetate | Clostridium sp. | |
2.8.3.9 | 0.167 | - |
crotonyl-CoA | pH 7.0, 25°C, cosubstrate acetoacetate | Clostridium sp. | |
2.8.3.9 | 0.205 | - |
propionyl-CoA | pH 7.0, 25°C, cosubstrate acetoacetate | Clostridium sp. | |
2.8.3.9 | 0.8 | - |
Butanoate | pH 7.5, 25°C | Clostridium sp. | |
2.8.3.9 | 1.1 | - |
acetoacetate | pH 7.5, 25°C, butyryl-CoA level of 0.18 mM | Clostridium sp. | |
2.8.3.9 | 1.4 | - |
acetoacetate | pH 7.5, 25°C, butyryl-CoA level of 0.23 mM | Clostridium sp. | |
2.8.3.9 | 1.8 | - |
acetoacetate | pH 7.5, 25°C, butyryl-CoA level of 0.37 mM | Clostridium sp. | |
2.8.3.9 | 2 | - |
crotonate | pH 7.5, 25°C | Clostridium sp. | |
2.8.3.9 | 3.2 | - |
propionate | pH 7.5, 25°C | Clostridium sp. | |
2.8.3.9 | 7 | - |
pentanoate | pH 7.5, 25°C | Clostridium sp. | |
2.8.3.9 | 14 | - |
Isobutyrate | pH 7.5, 25°C | Clostridium sp. | |
2.8.3.9 | 17 | - |
Vinylacetate | pH 7.5, 25°C | Clostridium sp. | |
2.8.3.9 | 21 | - |
Isopentanoate | pH 7.5, 25°C | Clostridium sp. | |
2.8.3.9 | 21 | - |
DL-2-Hydroxypentanoate | pH 7.5, 25°C | Clostridium sp. | |
2.8.3.9 | 24 | - |
acetate | pH 7.5, 25°C | Clostridium sp. | |
2.8.3.9 | 29 | - |
3-Mercaptopropionate | pH 7.5, 25°C | Clostridium sp. | |
2.8.3.9 | 29 | - |
Monochloroacetate | pH 7.5, 25°C | Clostridium sp. | |
2.8.3.9 | 33 | - |
4-hydroxybuyrate | pH 7.5, 25°C | Clostridium sp. | |
2.8.3.9 | 40 | - |
Acrylate | pH 7.5, 25°C | Clostridium sp. | |
2.8.3.9 | 42 | - |
3-Methylvinylacetate | pH 7.5, 25°C | Clostridium sp. | |
2.8.3.9 | 44 | - |
DL-3-hydroxybutyrate | pH 7.5, 25°C | Clostridium sp. | |
2.8.3.9 | 49 | - |
DL-2-hydroxybuyrate | pH 7.5, 25°C | Clostridium sp. | |
2.8.3.9 | 250 | - |
3-hydroxypropionate | pH 7.5, 25°C | Clostridium sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.8.3.9 | Ca2+ | activation at 6 mM CaCl2, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate | Clostridium sp. | |
2.8.3.9 | K+ | activation at 0.08 M KCl, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate | Clostridium sp. | |
2.8.3.9 | Li+ | activation at 0.2 M LiCl, inhibitions at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate | Clostridium sp. | |
2.8.3.9 | Mg2+ | activation at 12 mM MgCl2, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate | Clostridium sp. | |
2.8.3.9 | Mn2+ | activation at 1.2 mM MnCl2, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate | Clostridium sp. | |
2.8.3.9 | Na+ | activation at 0.17 M NaCl, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate | Clostridium sp. | |
2.8.3.9 | NH4+ | activation at 0.08 M NH4Cl, inhibition at higher concentrations, only with acetoacetate or acetoacetyl-CoA as substrate | Clostridium sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.8.3.9 | 23300 | - |
2 * 23300 + 2 * 25100, 2 alpha and 2 beta subunits, SDS-PAGE | Clostridium sp. |
2.8.3.9 | 25100 | - |
2 * 23300 + 2 * 25100, 2 alpha and 2 beta subunits, SDS-PAGE | Clostridium sp. |
2.8.3.9 | 90000 | - |
gel electrophoresis | Clostridium sp. |
2.8.3.9 | 108000 | - |
gel filtration | Clostridium sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.3.9 | butanoate + acetoacetyl-CoA | Acetoanaerobium sticklandii | - |
butanoyl-CoA + acetoacetate | - |
r | |
2.8.3.9 | butanoate + acetoacetyl-CoA | Clostridium sp. | lysine degradation pathway | butanoyl-CoA + acetoacetate | - |
r | |
2.8.3.9 | butanoate + acetoacetyl-CoA | Clostridium sp. SB4 | lysine degradation pathway | butanoyl-CoA + acetoacetate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.3.9 | Acetoanaerobium sticklandii | - |
- |
- |
2.8.3.9 | Clostridium sp. | - |
SB4 | - |
2.8.3.9 | Clostridium sp. SB4 | - |
SB4 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.8.3.9 | - |
Clostridium sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.8.3.9 | 329 | - |
- |
Clostridium sp. |
EC Number | Storage Stability | Organism |
---|---|---|
2.8.3.9 | -15°C 0.0062 mg/ml protein, 10 mM phosphate buffer, pH 7.0, half-life of 12 days when thawed and refrozen 5 times | Clostridium sp. |
2.8.3.9 | -15°C, 3.1 mg protein/ml, 20 mM potassium phosphate, pH 7.0, retains full activity during storage for 1 year | Clostridium sp. |
2.8.3.9 | 4°C, 0.0062 mg/ml protein, 10 mM phosphate buffer, pH 7.0, half-life of about 7 days | Clostridium sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.3.9 | 3-hydroxypropanoate + acetoacetyl-CoA | reaction at 8.6% the rate of butyrate | Clostridium sp. | 3-hydroxypropanoyl-CoA + acetoacetate | - |
r | |
2.8.3.9 | 3-mercaptopropionate + acetoacetyl-CoA | reaction at 71% the rate of butyrate | Clostridium sp. | 3-mercaptopropionyl-CoA + acetoacetate | - |
r | |
2.8.3.9 | 3-methylvinylacetate + acetoacetyl-CoA | reaction at 44% the rate of butyrate | Clostridium sp. | 3-methylvinylacetyl-CoA + acetoacetate | - |
r | |
2.8.3.9 | 4-hydroxybutyrate + acetoacetyl-CoA | reaction at 14% the rate of butyrate | Clostridium sp. | 4-hydroxybutanoyl-CoA + acetoacetate | - |
r | |
2.8.3.9 | acetate + acetoacetyl-CoA | - |
Clostridium sp. | acetyl-CoA + acetoacetate | - |
r | |
2.8.3.9 | acetate + acetoacetyl-CoA | reaction at 24% the rate of butyrate | Clostridium sp. | acetyl-CoA + acetoacetate | - |
r | |
2.8.3.9 | acetate + acetoacetyl-CoA | - |
Clostridium sp. SB4 | acetyl-CoA + acetoacetate | - |
r | |
2.8.3.9 | acetate + acetoacetyl-CoA | reaction at 24% the rate of butyrate | Clostridium sp. SB4 | acetyl-CoA + acetoacetate | - |
r | |
2.8.3.9 | acrylate + acetoacetyl-CoA | reaction at 10% the rate of butyrate | Clostridium sp. | acrylyl-CoA + acetoacetate | - |
r | |
2.8.3.9 | butanoate + acetoacetyl-CoA | - |
Acetoanaerobium sticklandii | butanoyl-CoA + acetoacetate | - |
r | |
2.8.3.9 | butanoate + acetoacetyl-CoA | - |
Clostridium sp. | butanoyl-CoA + acetoacetate | - |
r | |
2.8.3.9 | butanoate + acetoacetyl-CoA | lysine degradation pathway | Clostridium sp. | butanoyl-CoA + acetoacetate | - |
r | |
2.8.3.9 | butanoate + acetoacetyl-CoA | - |
Clostridium sp. SB4 | butanoyl-CoA + acetoacetate | - |
r | |
2.8.3.9 | butanoate + acetoacetyl-CoA | lysine degradation pathway | Clostridium sp. SB4 | butanoyl-CoA + acetoacetate | - |
r | |
2.8.3.9 | butanoyl-CoA + 2-methylacetoacetate | used 12% as fast as acetoacetate | Clostridium sp. | butanoate + 2-methylacetoacetyl-CoA | - |
r | |
2.8.3.9 | butanoyl-CoA + 3-oxohexanoate | used 21% as fast as acetoacetate | Clostridium sp. | butanoate + 3-oxohexanoyl-CoA | - |
r | |
2.8.3.9 | butanoyl-CoA + 3-oxopentanoate | used 60% as fast as acetoacetate | Clostridium sp. | butanoate + 3-oxopentanoyl-CoA | - |
r | |
2.8.3.9 | butanoyl-CoA + acetoacetate | - |
Clostridium sp. | butanoate + acetoacetyl-CoA | - |
r | |
2.8.3.9 | crotonate + acetoacetyl-CoA | reaction at 16% the rate of butyrate | Clostridium sp. | crotonyl-CoA + acetoacetate | - |
r | |
2.8.3.9 | DL-2-hydroxybutyrate + acetoacetyl-CoA | reaction at 19% the rate of butyrate | Clostridium sp. | 2-hydroxybutyryl-CoA + acetoacetate | - |
r | |
2.8.3.9 | DL-2-hydroxypentanoate + acetoacetyl-CoA | reaction at 7.9% the rate of butyrate | Clostridium sp. | 2-hydroxypentanoyl-CoA + acetoacetate | - |
r | |
2.8.3.9 | DL-3-hydroxybutyrate + acetoacetyl-CoA | reaction at 30% the rate of butyrate | Clostridium sp. | 3-hydroxybutyryl-CoA + acetoacetate | - |
r | |
2.8.3.9 | isobutyrate + acetoacetyl-CoA | reaction at 19% the rate of butyrate | Clostridium sp. | isobutyryl-CoA + acetoacetate | - |
r | |
2.8.3.9 | isopentanoate + acetoacetyl-CoA | reaction at 8.3% the rate of butyrate | Clostridium sp. | isopentanoyl-CoA + acetoacetate | - |
r | |
2.8.3.9 | monochloroacetate + acetoacetyl-CoA | reaction at 5.4% the rate of butyrate | Clostridium sp. | monochloroacetyl-CoA + acetoacetate | - |
r | |
2.8.3.9 | additional information | monofluoroacetate and hexanoate are no substrates, compounds found to be unreactive or less than 0.1% as reactive as butyrate are formate, cyanoacetate, n-heptanoate, tiglate, oxalate, malonate, succinate, citramalate, DL-alpha-hydroxyisobutyrate, 3,4-dihydroxybutyrate, alpha-ketobutyrate, 2-oxoglutarate, DL-3-aminobutyrate and 4-aminobutyrate, 3-oxo-5-aminohexanoate and triacetate are unreactive | Clostridium sp. | ? | - |
? | |
2.8.3.9 | additional information | monofluoroacetate and hexanoate are no substrates, compounds found to be unreactive or less than 0.1% as reactive as butyrate are formate, cyanoacetate, n-heptanoate, tiglate, oxalate, malonate, succinate, citramalate, DL-alpha-hydroxyisobutyrate, 3,4-dihydroxybutyrate, alpha-ketobutyrate, 2-oxoglutarate, DL-3-aminobutyrate and 4-aminobutyrate, 3-oxo-5-aminohexanoate and triacetate are unreactive | Clostridium sp. SB4 | ? | - |
? | |
2.8.3.9 | pentanoate + acetoacetyl-CoA | reaction at 19% the rate of butyrate | Clostridium sp. | pentanoyl-CoA + acetoacetate | - |
r | |
2.8.3.9 | propionate + acetoacetyl-CoA | reaction at 21% the rate of butyrate | Clostridium sp. | propionyl-CoA + acetoacetate | - |
r | |
2.8.3.9 | vinylacetate + acetoacetyl-CoA | best substrate | Clostridium sp. | vinylacetyl-CoA + acetoacetate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.8.3.9 | tetramer | 2 * 23300 + 2 * 25100, 2 alpha and 2 beta subunits, SDS-PAGE | Clostridium sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.8.3.9 | 30 | - |
1 h, in potassium phosphate buffer, pH 6.5-7.0, most stable, about 20% loss of activity at pH 6.0 and 8.0 | Clostridium sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.8.3.9 | 6.5 | 8 | - |
Clostridium sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.8.3.9 | 4 | 9 | maximally active from pH 6.6 to pH 7.9 | Clostridium sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.8.3.9 | 6.5 | 7 | 1 h, at 30°C, most stable, about 20% loss of activity at pH 6.0 and 8.0 | Clostridium sp. |