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Literature summary extracted from

  • Jain, J.C.; Reed, D.W.; Groot Wassink, J.W.D.; Underhill, E.W.
    A radioassay of enzymes catalyzing the glucosylation and sulfation steps of glucosinolate biosynthesis in Brassica species (1989), Anal. Biochem., 178, 137-140.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.195 UDPglucose + phenylacetothiohydroximate Brassica napus
-
 UDP + desulfoglucotropeolin
-
 ?
2.4.1.195 UDPglucose + phenylacetothiohydroximate Brassica oleracea
-
 UDP + desulfoglucotropeolin
-
 ?
2.4.1.195 UDPglucose + phenylacetothiohydroximate Brassica rapa subsp. oleifera
-
 UDP + desulfoglucotropeolin
-
 ?
2.4.1.195 UDPglucose + phenylacetothiohydroximate Brassica juncea biosynthetic pathway for glucosinolates UDP + desulfoglucotropeolin
-
 ?
2.4.1.195 UDPglucose + phenylacetothiohydroximate Brassica nigra biosynthetic pathway for glucosinolates UDP + desulfoglucotropeolin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.195 Brassica juncea
-
 cv. Cutlass
-
2.4.1.195 Brassica juncea
-
 cv Domo
-
2.4.1.195 Brassica napus
-
 cv Westar
-
2.4.1.195 Brassica nigra
-
 cv 526
-
2.4.1.195 Brassica oleracea
-
 savoy cabbage
-
2.4.1.195 Brassica rapa subsp. oleifera
-
 cv R-500
-
2.8.2.24 Brassica juncea
-
 cv. Domo
-
2.8.2.24 Brassica juncea
-
 cv. Cutlass
-
2.8.2.24 Brassica napus
-
 rapeseed, cv. Westar
-
2.8.2.24 Brassica nigra
-
 cv. 526
-
2.8.2.24 Brassica oleracea
-
 savoy cabbage
-
2.8.2.24 Brassica rapa subsp. oleifera
-
 cv. R-500
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.195
-
 Brassica napus
2.8.2.24 partial Brassica napus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.195 seedling
-
 Brassica napus
-
2.4.1.195 seedling
-
 Brassica oleracea
-
2.4.1.195 seedling
-
 Brassica juncea
-
2.8.2.24 seedling
-
 Brassica napus
-
2.8.2.24 seedling
-
 Brassica oleracea
-
2.8.2.24 seedling
-
 Brassica rapa subsp. oleifera
-
2.8.2.24 seedling
-
 Brassica juncea
-
2.8.2.24 seedling
-
 Brassica nigra
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.2.24 additional information
-
-
 Brassica napus
2.8.2.24 additional information
-
-
 Brassica oleracea
2.8.2.24 additional information
-
-
 Brassica rapa subsp. oleifera
2.8.2.24 additional information
-
-
 Brassica juncea
2.8.2.24 additional information
-
-
 Brassica nigra

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.195 UDPglucose + phenylacetothiohydroximate
-
 Brassica napus UDP + desulfoglucotropeolin
-
 ?
2.4.1.195 UDPglucose + phenylacetothiohydroximate
-
 Brassica oleracea UDP + desulfoglucotropeolin
-
 ?
2.4.1.195 UDPglucose + phenylacetothiohydroximate
-
 Brassica rapa subsp. oleifera UDP + desulfoglucotropeolin
-
 ?
2.4.1.195 UDPglucose + phenylacetothiohydroximate
-
 Brassica juncea UDP + desulfoglucotropeolin
-
 ?
2.4.1.195 UDPglucose + phenylacetothiohydroximate
-
 Brassica nigra UDP + desulfoglucotropeolin
-
 ?
2.4.1.195 UDPglucose + phenylacetothiohydroximate biosynthetic pathway for glucosinolates Brassica juncea UDP + desulfoglucotropeolin
-
 ?
2.4.1.195 UDPglucose + phenylacetothiohydroximate biosynthetic pathway for glucosinolates Brassica nigra UDP + desulfoglucotropeolin
-
 ?
2.8.2.24 3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate absolute specificity for desulfoglucosinolate structure Brassica napus adenosine 3',5'-bisphosphate + benzylglucosinolate
-
 ?
2.8.2.24 3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate absolute specificity for desulfoglucosinolate structure Brassica oleracea adenosine 3',5'-bisphosphate + benzylglucosinolate
-
 ?
2.8.2.24 3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate absolute specificity for desulfoglucosinolate structure Brassica rapa subsp. oleifera adenosine 3',5'-bisphosphate + benzylglucosinolate
-
 ?
2.8.2.24 3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate absolute specificity for desulfoglucosinolate structure Brassica juncea adenosine 3',5'-bisphosphate + benzylglucosinolate
-
 ?
2.8.2.24 3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate absolute specificity for desulfoglucosinolate structure Brassica nigra adenosine 3',5'-bisphosphate + benzylglucosinolate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.195 uridine diphosphoglucose (UDPglucose):thiohydroximate glucosyltransferase
-
 Brassica nigra