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Literature summary extracted from

  • Nussbaum, J.L.; Mandel, P.
    Enzymic synthesis of psychosine sulphate (1972), J. Neurochem., 19, 1789-1802.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.2.13 2-mercaptoethanol
-
 Mus musculus
2.8.2.13 cysteine
-
 Mus musculus
2.8.2.13 deoxycholate
-
 Mus musculus
2.8.2.13 EDTA stimulatory effect Mus musculus
2.8.2.13 glutathione stimulates SO42- incorporation by 23% Mus musculus
2.8.2.13 Tween 20 detergent is necessary for enzyme action in vitro, removing Tween 20 from the incubation medium inhibits enzyme activity 92.5% Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.2.13 ATP decreases the incorporation of sulfate ions into psychosine sulfate Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.13 0.5
-
 galactosylsphingosine pH 7.4, 37°C, psychosine Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.2.13 cytosol
-
 Mus musculus 5829
-
2.8.2.13 microsome
-
 Mus musculus
-
-
2.8.2.13 mitochondrion
-
 Mus musculus 5739
-
2.8.2.13 synaptosome
-
 Mus musculus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.2.13 Mg2+ divalent ions other than magnesium causes a reduction of enzyme activity Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.13 3'-phosphoadenylylsulfate + galactosylsphingosine Mus musculus
-
 adenosine 3',5'-bisphosphate + psychosine sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.13 Mus musculus
-
 mouse, normal and Jimpy mutant, maximum enzyme activity at 17-19 days after birth
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.13 brain
-
 Mus musculus
-
2.8.2.13 kidney
-
 Mus musculus
-
2.8.2.13 additional information hardly any effect in liver Mus musculus
-

Storage Stability

EC Number Storage Stability Organism
2.8.2.13 -20°C, microsomes or whole homogenate retains enzyme activity for at least 1 month Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.13 3'-phosphoadenylylsulfate + galactosylsphingosine
-
 Mus musculus adenosine 3',5'-bisphosphate + psychosine sulfate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.8.2.13 45 55 at 45°C enzyme activity is reduced by 85%, at 55°C activity is lost Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.13 6.8
-
-
 Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.8.2.13 6.4 8 about 50% of activity maximum at pH 6.4, about 60% of activity maximum at pH 7.4 Mus musculus