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Literature summary extracted from

  • Wong, T.W.; Weiss, S.B.; Eliceiri, G.L.; Bryant, J.
    Ribonucleic acid sulfurtransferase from Bacillus subtilis W168. Sulfuration with beta-mercaptopyruvate and properties of the enzyme system (1970), Biochemistry, 9, 2376-2386.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.1.4 3-Mercaptopyruvate competitive inhibitor, supresses the transfer of sulfur to tRNA Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.4 0.0069
-
 3-Mercaptopyruvate pH 7.8, 37°C Bacillus subtilis
2.8.1.4 1
-
 L-cysteine pH 7.8, 37°C Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.1.4 Mg2+
-
 Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.4 L-cysteine + 'activated' tRNA Bacillus subtilis
-
 L-serine + tRNA containing a thionucleotide
-
 r
2.8.1.4 L-cysteine + 'activated' tRNA Bacillus subtilis W168
-
 L-serine + tRNA containing a thionucleotide
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.4 Bacillus subtilis
-
 W168
-
2.8.1.4 Bacillus subtilis W168
-
 W168
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.4 partially Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.1.4 0.00815
-
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.4 3-mercaptopyruvate + activated tRNA
-
 Bacillus subtilis ? + S-tRNA
-
 ?
2.8.1.4 L-cysteine + 'activated' tRNA
-
 Bacillus subtilis L-serine + tRNA containing a thionucleotide
-
 r
2.8.1.4 L-cysteine + 'activated' tRNA
-
 Bacillus subtilis W168 L-serine + tRNA containing a thionucleotide
-
 r
2.8.1.4 L-cysteine + Bacillus subtilis 'activated' tRNA
-
 Bacillus subtilis L-serine + tRNA containing a thionucleotide
-
 r
2.8.1.4 L-cysteine + Bacillus subtilis 'activated' tRNA
-
 Bacillus subtilis W168 L-serine + tRNA containing a thionucleotide
-
 r
2.8.1.4 L-cysteine + denatured DNA DNA prepared from various sources appears to function as a good sulfur acceptor Bacillus subtilis L-serine + ?
-
 ?
2.8.1.4 L-cysteine + E. coli 'activated' tRNA
-
 Bacillus subtilis L-serine + tRNA containing a thionucleotide
-
 r
2.8.1.4 L-cysteine + E. coli 'activated' tRNA
-
 Bacillus subtilis W168 L-serine + tRNA containing a thionucleotide
-
 r
2.8.1.4 L-cysteine + Rattus norvegicus liver 'activated' tRNA
-
 Bacillus subtilis L-serine + tRNA containing a thionucleotide
-
 r
2.8.1.4 L-cysteine + Saccharomyces cerevisiae 'activated' tRNA
-
 Bacillus subtilis L-serine + tRNA containing a thionucleotide
-
 r
2.8.1.4 L-cysteine + Saccharomyces cerevisiae 'activated' tRNA
-
 Bacillus subtilis W168 L-serine + tRNA containing a thionucleotide
-
 r
2.8.1.4 additional information only transfer RNA as sulfur acceptor, other cellular species of RNA and synthetic ribohomopolymers do not accept sulfur in this system, no reaction with E. coli rRNA, phage MS2 RNA, polyC, polyA, or polyU Bacillus subtilis ?
-
 ?
2.8.1.4 additional information only transfer RNA as sulfur acceptor, other cellular species of RNA and synthetic ribohomopolymers do not accept sulfur in this system, no reaction with E. coli rRNA, phage MS2 RNA, polyC, polyA, or polyU Bacillus subtilis W168 ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.1.4 ATP absolute requirement, other nucleotides are inactive Bacillus subtilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.1.4 0.005
-
 3-Mercaptopyruvate pH 7.8, 37°C Bacillus subtilis