Literature summary extracted from

Rilfors, L.; Niemi, A.; Haraldsson, S.; Edwards, K.; Andersson, A.S.; Dowhan, W.
Reconstituted phosphatidylserine synthase from Escherichia coli is activated by anionic phospholipids and micelle-forming amphiphiles (1999), Biochim. Biophys. Acta, 1438, 281-294.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
2.7.8.8	additional information	the enzyme reconstituted with lipid vesicles of various compositions exhibits practically no activity in the absence of a detergent and with the substrate CDP-diacylglycerol present only in the lipid vesicles. Inclusion of octylglucoside in the assay mixture increases the activity 20- to 1000fold, the degree of activation depends on the lipid composition of the vesicles. Inclusion of additional CDP-diacylglycerol in the assay mixture increases the activity 5- to 25-fold. When the fraction of phosphatidylglycerol is increased from 15 to 100 mol% in the vesicles the activity increases 10fold using the assay mixture containing octylglucoside. The highest activities are exhibited with the anionic lipids diphosphatidylglycerol and phosphatidic acid while phosphatidylinositol gives lower activity	Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.7.8.8	CDP-diacylglycerol + L-serine	Escherichia coli	increase in activity caused by phosphatidylglycerol and diphosphatidylglycerol is physiologically relevant. It may be part of a regulatory mechanism that keeps the balance between phosphatidylethanolamine and the sum of phosphatidylglycerol and diphosphatidylglycerol	CMP + 3-O-sn-phosphatidyl-L-serine	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.8.8	Escherichia coli	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.8.8	CDP-diacylglycerol + L-Ser	-	Escherichia coli	CMP + 3-O-sn-phosphatidyl-L-serine	-	?
2.7.8.8	CDP-diacylglycerol + L-serine	increase in activity caused by phosphatidylglycerol and diphosphatidylglycerol is physiologically relevant. It may be part of a regulatory mechanism that keeps the balance between phosphatidylethanolamine and the sum of phosphatidylglycerol and diphosphatidylglycerol	Escherichia coli	CMP + 3-O-sn-phosphatidyl-L-serine	-	?

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Release 2023.1 (January 2023)