BRENDA - Enzyme Database

Phosphatidylserine synthase from Escherichia coli

Dowhan, W.; Larson, T.; Methods Enzymol. 71, 561-571 (1981)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.8
additional information
optimal activity is dependent on ionic strength, 0.3 or higher
Escherichia coli
2.7.8.8
Triton X-100
enzyme is dependent on a nonionic detergent such as Triton X-100
Escherichia coli
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.8
ribosome
tightly bound to ribosome
Escherichia coli
5840
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.8.8
54000
-
x * 54000, SDS-PAGE
Escherichia coli
2.7.8.8
500000
-
glycerol gradient centrifugation
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.8
Escherichia coli
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.8.8
-
Escherichia coli
Reaction
EC Number
Reaction
Commentary
Organism
2.7.8.8
CDP-diacylglycerol + L-serine = CMP + (3-sn-phosphatidyl)-L-serine
the enzyme appears to catalyze a ping-pong reaction
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.8.8
39
-
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.8
CDP-1,2-dicaproyl-DL-glycerol + L-Ser
-
643778
Escherichia coli
CMP + 3-O-sn-1,2-dicaproylphosphatidylserine
-
-
-
?
2.7.8.8
CDP-1,2-dipalmitoyl-L-glycerol + L-Ser
-
643778
Escherichia coli
CMP + 1,2-dipalmitoylphosphatidylserine
-
-
-
?
2.7.8.8
CDP-diacylglycerol + H2O
at 1% of the synthetic rate the enzyme catalyzes the hydrolysis of phosphatidylserine to CMP and phosphatidic acid
643778
Escherichia coli
CMP + phosphatidic acid
-
643778
Escherichia coli
-
2.7.8.8
CDP-diacylglycerol + L-Ser
-
643778
Escherichia coli
CMP + 3-O-sn-phosphatidyl-L-serine
-
643778
Escherichia coli
?
2.7.8.8
additional information
the enzyme also catalyzes the exchange reaction between Ser and phosphatidylserine
643778
Escherichia coli
?
-
-
-
-
2.7.8.8
additional information
enzyme catalyzes exchange reaction between CMP and CDP-diglyceride
643778
Escherichia coli
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.7.8.8
?
x * 54000, SDS-PAGE
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.8
7
8.5
-
Escherichia coli
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.8
additional information
optimal activity is dependent on ionic strength, 0.3 or higher
Escherichia coli
2.7.8.8
Triton X-100
enzyme is dependent on a nonionic detergent such as Triton X-100
Escherichia coli
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.8
ribosome
tightly bound to ribosome
Escherichia coli
5840
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.8.8
54000
-
x * 54000, SDS-PAGE
Escherichia coli
2.7.8.8
500000
-
glycerol gradient centrifugation
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.8.8
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.8.8
39
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.8
CDP-1,2-dicaproyl-DL-glycerol + L-Ser
-
643778
Escherichia coli
CMP + 3-O-sn-1,2-dicaproylphosphatidylserine
-
-
-
?
2.7.8.8
CDP-1,2-dipalmitoyl-L-glycerol + L-Ser
-
643778
Escherichia coli
CMP + 1,2-dipalmitoylphosphatidylserine
-
-
-
?
2.7.8.8
CDP-diacylglycerol + H2O
at 1% of the synthetic rate the enzyme catalyzes the hydrolysis of phosphatidylserine to CMP and phosphatidic acid
643778
Escherichia coli
CMP + phosphatidic acid
-
643778
Escherichia coli
-
2.7.8.8
CDP-diacylglycerol + L-Ser
-
643778
Escherichia coli
CMP + 3-O-sn-phosphatidyl-L-serine
-
643778
Escherichia coli
?
2.7.8.8
additional information
the enzyme also catalyzes the exchange reaction between Ser and phosphatidylserine
643778
Escherichia coli
?
-
-
-
-
2.7.8.8
additional information
enzyme catalyzes exchange reaction between CMP and CDP-diglyceride
643778
Escherichia coli
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.8.8
?
x * 54000, SDS-PAGE
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.8
7
8.5
-
Escherichia coli