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Literature summary extracted from

  • Dowhan, W.; Larson, T.
    Phosphatidylserine synthase from Escherichia coli (1981), Methods Enzymol., 71, 561-571.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.8.8 additional information optimal activity is dependent on ionic strength, 0.3 or higher Escherichia coli
2.7.8.8 Triton X-100 enzyme is dependent on a nonionic detergent such as Triton X-100 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.8 ribosome tightly bound to ribosome Escherichia coli 5840
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.8 54000
-
 x * 54000, SDS-PAGE Escherichia coli
2.7.8.8 500000
-
 glycerol gradient centrifugation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.8 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.8
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.8.8 CDP-diacylglycerol + L-serine = CMP + (3-sn-phosphatidyl)-L-serine the enzyme appears to catalyze a ping-pong reaction Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.8 39
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.8 CDP-1,2-dicaproyl-DL-glycerol + L-Ser
-
 Escherichia coli CMP + 3-O-sn-1,2-dicaproylphosphatidylserine
-
 ?
2.7.8.8 CDP-1,2-dipalmitoyl-L-glycerol + L-Ser
-
 Escherichia coli CMP + 1,2-dipalmitoylphosphatidylserine
-
 ?
2.7.8.8 CDP-diacylglycerol + H2O at 1% of the synthetic rate the enzyme catalyzes the hydrolysis of phosphatidylserine to CMP and phosphatidic acid Escherichia coli CMP + phosphatidic acid
-
 ?
2.7.8.8 CDP-diacylglycerol + L-Ser
-
 Escherichia coli CMP + 3-O-sn-phosphatidyl-L-serine
-
 ?
2.7.8.8 additional information the enzyme also catalyzes the exchange reaction between Ser and phosphatidylserine Escherichia coli ?
-
 ?
2.7.8.8 additional information enzyme catalyzes exchange reaction between CMP and CDP-diglyceride Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.8.8 ? x * 54000, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.8 7 8.5
-
 Escherichia coli