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Literature summary extracted from

  • Martz, F.; Wilczynska, M.; Kleczkowski, L.A.
    Oligomerization status, with the monomer as active species, defines catalytic efficiency of UDP-glucose pyrophosphorylase (2002), Biochem. J., 367, 295-300.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.9 gene Ugp, expression in Escherichia coli Hordeum vulgare

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.9 C99S site-directed mutagenesis, half Vmax of wild-type, 12fold higher Km for diphosphate, altered diphosphate binding Hordeum vulgare
2.7.7.9 additional information amino acid exchanges in hydrophobic domain Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.9 0.034
-
 diphosphate recombinant wild-type, pH 7.6, 25°C Hordeum vulgare
2.7.7.9 0.065
-
 UDP-glucose recombinant wild-type, pH 7.6, 25°C Hordeum vulgare
2.7.7.9 0.101
-
 UDP-glucose recombinant mutant C99S, pH 7.6, 25°C Hordeum vulgare
2.7.7.9 0.407
-
 diphosphate recombinant mutant C99S, pH 7.6, 25°C Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.9 additional information
-
 mixture of monomers, dimers and higher-order polymers, native PAGE and gel filtration, the monomer is the active form Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.9 UTP + alpha-D-glucose 1-phosphate Hordeum vulgare key enzyme in biosynthesis of sucrose, cellulose, and other saccharides diphosphate + UDP-glucose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.9 Hordeum vulgare Q43772 gene Ugp
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.9 native and recombinant wild-type, the latter from Escherichia coli to homogeneity Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.9 leaf
-
 Hordeum vulgare
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.9 UTP + alpha-D-glucose 1-phosphate
-
 Hordeum vulgare diphosphate + UDP-glucose
-
 r
2.7.7.9 UTP + alpha-D-glucose 1-phosphate key enzyme in biosynthesis of sucrose, cellulose, and other saccharides Hordeum vulgare diphosphate + UDP-glucose
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.7.9 UGPase
-
 Hordeum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.9 25
-
 assay at Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.9 7.6
-
 assay at Hordeum vulgare