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Literature summary extracted from

  • Rudick, V.L.; Weisman, R.A.
    Uridine diphosphate glucose pyrophosphorylase of Acanthamoeba castellanii. Purification, kinetic, and developmental studies (1974), J. Biol. Chem., 249, 7832-7840.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.9 diphosphate product inhibition Acanthamoeba castellanii
2.7.7.9 UTP product inhibition Acanthamoeba castellanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.9 0.05 0.066 UDP-glucose pH 7.6, 37°C Acanthamoeba castellanii
2.7.7.9 0.058 0.07 UTP pH 8.0, 37°C Acanthamoeba castellanii
2.7.7.9 0.14 0.18 alpha-D-glucose 1-phosphate
-
 Acanthamoeba castellanii
2.7.7.9 0.68 1 alpha-D-glucose 1-phosphate pH 8.0, 37°C Acanthamoeba castellanii
2.7.7.9 2.4
-
 diphosphate pH 7.6, 37°C Acanthamoeba castellanii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.9 Ca2+ activation Acanthamoeba castellanii
2.7.7.9 Co2+ activation Acanthamoeba castellanii
2.7.7.9 Mg2+ requirement Acanthamoeba castellanii
2.7.7.9 Mg2+ MgUTP is the actual substrate Acanthamoeba castellanii
2.7.7.9 Mg2+ Mg-diphosphate is the actual substrate Acanthamoeba castellanii
2.7.7.9 additional information requirement for divalent cations Acanthamoeba castellanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.9 UTP + alpha-D-glucose 1-phosphate Acanthamoeba castellanii enzyme is induced during amoeba encystment diphosphate + UDP-glucose
-
 r
2.7.7.9 UTP + alpha-D-glucose 1-phosphate Acanthamoeba castellanii NEFF enzyme is induced during amoeba encystment diphosphate + UDP-glucose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.9 Acanthamoeba castellanii
-
 Neff
-
2.7.7.9 Acanthamoeba castellanii NEFF
-
 Neff
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.9
-
 Acanthamoeba castellanii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.9 cyst
-
 Acanthamoeba castellanii
-
2.7.7.9 cyst enzyme activity increases during encystment Acanthamoeba castellanii
-
2.7.7.9 trophozoite
-
 Acanthamoeba castellanii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.9 15.8
-
 purified enzyme Acanthamoeba castellanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.9 diphosphate + CDP-glucose no activity Acanthamoeba castellanii CTP + alpha-D-glucose 1-phosphate
-
 ?
2.7.7.9 diphosphate + GDP-glucose no activity Acanthamoeba castellanii GTP + alpha-D-glucose 1-phosphate
-
 ?
2.7.7.9 diphosphate + TDP-glucose no activity Acanthamoeba castellanii TTP + alpha-D-glucose 1-phosphate
-
 ?
2.7.7.9 additional information no activity with ADP-glucose Acanthamoeba castellanii ?
-
 ?
2.7.7.9 additional information no activity with ATP, CTP, and GTP Acanthamoeba castellanii ?
-
 ?
2.7.7.9 additional information no activity with ADP-glucose Acanthamoeba castellanii NEFF ?
-
 ?
2.7.7.9 additional information no activity with ATP, CTP, and GTP Acanthamoeba castellanii NEFF ?
-
 ?
2.7.7.9 UTP + alpha-D-glucose 1-phosphate highly specific for UTP Acanthamoeba castellanii diphosphate + UDP-glucose specific for UDP-glucose r
2.7.7.9 UTP + alpha-D-glucose 1-phosphate reverse reaction: highly specific for UDP-glucose Acanthamoeba castellanii diphosphate + UDP-glucose specific for UDP-glucose r
2.7.7.9 UTP + alpha-D-glucose 1-phosphate enzyme is induced during amoeba encystment Acanthamoeba castellanii diphosphate + UDP-glucose
-
 r
2.7.7.9 UTP + alpha-D-glucose 1-phosphate highly specific for UTP Acanthamoeba castellanii NEFF diphosphate + UDP-glucose specific for UDP-glucose r
2.7.7.9 UTP + alpha-D-glucose 1-phosphate reverse reaction: highly specific for UDP-glucose Acanthamoeba castellanii NEFF diphosphate + UDP-glucose specific for UDP-glucose r
2.7.7.9 UTP + alpha-D-glucose 1-phosphate enzyme is induced during amoeba encystment Acanthamoeba castellanii NEFF diphosphate + UDP-glucose
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.9 37
-
 assay at Acanthamoeba castellanii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.9 37
-
 30 min, stable Acanthamoeba castellanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.9 7.5 7.6 pyrophosphorolysis Acanthamoeba castellanii
2.7.7.9 8 9 UDP-glucose synthesis Acanthamoeba castellanii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.9 7.2 9.8 about half-maximal activity at pH 7.2 and about 90% of maximal activity at pH 9.8 Acanthamoeba castellanii