Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Simuth, J.; Zelinka, J.S.; Polek, B.
    Polynucleotide phosphorylase from Streptomyces aureofaciens: purification and properties (1975), Biochim. Biophys. Acta, 379, 397-407.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.8 cytidyldadenylate dinucleoside activates GDP polymerization Kitasatospora aureofaciens
2.7.7.8 KCl 400 mM, 5fold activation of ADP polymerization Kitasatospora aureofaciens
2.7.7.8 NaCl 400 mM, 4fold activation of ADP polymerization Kitasatospora aureofaciens
2.7.7.8 NH4+ 400 mM, 3 and 7fold activation of ADP polymerization and poly(A) phosphorolysis, respectively Kitasatospora aureofaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.8 chlortetracycline competitive inhibition of ADP polymerization Kitasatospora aureofaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.8 1
-
 ADP approx. value, also for CDP and UDP polymerization Kitasatospora aureofaciens
2.7.7.8 1
-
 ADP pH 9.5, 37°C, approx. value Kitasatospora aureofaciens
2.7.7.8 1
-
 UDP pH 9.5, 37°C, approx. value Kitasatospora aureofaciens
2.7.7.8 1
-
 CDP pH 9.5, 37°C, approx. value Kitasatospora aureofaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.8 Ca2+ activity depends on divalent cation, efficiency in descending order: Mg2+, Mn2+, Co2+, Zn2+, Cu2+, Ca2+ Kitasatospora aureofaciens
2.7.7.8 Co2+ activity depends on divalent cation, efficiency in descending order: Mg2+, Mn2+, Co2+, Zn2+, Cu2+, Ca2+ Kitasatospora aureofaciens
2.7.7.8 Cu2+ activity depends on divalent cation, efficiency in descending order: Mg2+, Mn2+, Co2+, Zn2+, Cu2+, Ca2+ Kitasatospora aureofaciens
2.7.7.8 K+
-
 Kitasatospora aureofaciens
2.7.7.8 Mg2+ activity depends on divalent cation, order of efficiency: Mg2+, Mn2+, Co2+, Zn2+, Cu2+, Ca2+ Kitasatospora aureofaciens
2.7.7.8 Mn2+ activity depends on divalent cation, efficiency in descending order: Mg2+, Mn2+, Co2+, Zn2+, Cu2+, Ca2+ Kitasatospora aureofaciens
2.7.7.8 Na+
-
 Kitasatospora aureofaciens
2.7.7.8 Zn2+ activity depends on divalent cation, efficiency in descending order: Mg2+, Mn2+, Co2+, Zn2+, Cu2+, Ca2+ Kitasatospora aureofaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.8 100000
-
 2 * 100000, SDS-PAGE in the presence of 2-mercaptoethanol Kitasatospora aureofaciens
2.7.7.8 210000
-
 low speed sedimentation equilibrium Kitasatospora aureofaciens

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.8 Kitasatospora aureofaciens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.8 polymin P, DEAE-cellulose, Sephadex G-150, DEAE-cellulose, Sephadex G-200 Kitasatospora aureofaciens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.8 35.8
-
-
 Kitasatospora aureofaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.8 poly(A) + ADP
-
 Kitasatospora aureofaciens poly(A)+1 + phosphate
-
 r
2.7.7.8 poly(C) + CDP
-
 Kitasatospora aureofaciens poly(C)+1 + phosphate
-
 r
2.7.7.8 poly(U)+ UDP
-
 Kitasatospora aureofaciens poly(U)+1 + phosphate
-
 r
2.7.7.8 poly(U)+1 + phosphate
-
 Kitasatospora aureofaciens poly(U) + ADP
-
 r
2.7.7.8 ribonucleoside 5'-diphosphate + phosphate
-
 Kitasatospora aureofaciens ribonucleoside 5'-diphosphate + phosphate exchange reaction ?
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of CDP Kitasatospora aureofaciens RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of UDP Kitasatospora aureofaciens RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of ADP Kitasatospora aureofaciens RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + UDP
-
 Kitasatospora aureofaciens RNAn+1 + phosphate
-
 r
2.7.7.8 RNAn+1 + phosphate phosphorolysis of poly(U) Kitasatospora aureofaciens RNAn + a nucleoside diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.8 dimer 2 * 100000, SDS-PAGE in the presence of 2-mercaptoethanol Kitasatospora aureofaciens