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Literature summary extracted from

  • Erickson, R.J.; Grosch, J.C.
    Isolation and characterization of a polynucleotide phosphorylase from Bacillus amyloliquefaciens (1977), J. Bacteriol., 130, 869-876.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.8 Mg2+ stimulates ADP polymerization maximally at 10 mM, GDP polymerization maximally at 5 mM, inhibition above Bacillus amyloliquefaciens
2.7.7.8 additional information relatively insensitive to N-ethylmaleimide and high concentrations of KCl Bacillus amyloliquefaciens
2.7.7.8 phosphate complete inhibition of polymerization at low concentrations Bacillus amyloliquefaciens
2.7.7.8 Poly(A) inhibition of poly(A) synthesis Bacillus amyloliquefaciens
2.7.7.8 Poly(G) inhibition of poly(A) synthesis Bacillus amyloliquefaciens
2.7.7.8 rifamycin SV partial inhibition of polymerization Bacillus amyloliquefaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.8 Mg2+ required for activity Bacillus amyloliquefaciens
2.7.7.8 Mg2+ maximal activation of ADP and GDP polymerization at 10 and 5 mM, respectively, inhibition at higher concentrations Bacillus amyloliquefaciens
2.7.7.8 Mn2+ can partially replace Mg2+ in activation Bacillus amyloliquefaciens

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.8 Bacillus amyloliquefaciens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.8 phase partition, ammonium sulfate, agarose gel filtration, DEAE-Sephadex Bacillus amyloliquefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.8 poly(A) + ADP
-
 Bacillus amyloliquefaciens poly(A)+1 + phosphate
-
 r
2.7.7.8 poly(G) + GDP
-
 Bacillus amyloliquefaciens poly(G)+1 + phosphate
-
 r
2.7.7.8 ribonucleoside 5'-diphosphate + phosphate
-
 Bacillus amyloliquefaciens ribonucleoside 5'-diphosphate + phosphate exchange reaction ?
2.7.7.8 RNAn + a nucleoside diphosphate primer-independent activity Bacillus amyloliquefaciens RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of GDP Bacillus amyloliquefaciens RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of ADP Bacillus amyloliquefaciens RNAn+1 + phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.8 5
-
 polymerization of ADP Bacillus amyloliquefaciens
2.7.7.8 7 9 polymerization of GDP Bacillus amyloliquefaciens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.8 5 9.5 almost no activity below Bacillus amyloliquefaciens