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Literature summary extracted from

  • Waters, M.F.; McLennan, A.G.; Carr, N.G.
    Purification and activity gel analysis of polynucleotide phosphorylase from the cyanobacterium Nostoc sp. MAC (1989), J. Gen. Microbiol., 135, 2045-2054.
No PubMed abstract available

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.8 Mg2+ required for activity Nostoc sp.
2.7.7.8 Mn2+ 7-9% of activity with Mg2+ Nostoc sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.8 91000
-
 alpha3, 3 * 91000, SDS-PAGE Nostoc sp.
2.7.7.8 215000 240000 gel filtration Nostoc sp.
2.7.7.8 240000
-
 sedimentation equilibrium Nostoc sp.

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.8 Nostoc sp.
-
 MAC
-
2.7.7.8 Nostoc sp. MAC
-
 MAC
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.8 DEAE-cellulose, Sephacryl S-300, Mono Q, poly(A)-Sepharose Nostoc sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.8 2
-
 at pH 10.5 Nostoc sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.8 poly(A) + ADP
-
 Nostoc sp. poly(A)+1 + phosphate
-
 r
2.7.7.8 poly(A) + ADP
-
 Nostoc sp. MAC poly(A)+1 + phosphate
-
 r
2.7.7.8 poly(U)+ UDP
-
 Nostoc sp. poly(U)+1 + phosphate
-
 r
2.7.7.8 RNAn + a nucleoside diphosphate primer-independent activity Nostoc sp. RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of UDP Nostoc sp. RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of ADP Nostoc sp. RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate purified enzyme is less dependent on a primer than the enzyme in crude extracts Nostoc sp. RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate primer-independent activity Nostoc sp. MAC RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of UDP Nostoc sp. MAC RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of ADP Nostoc sp. MAC RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate purified enzyme is less dependent on a primer than the enzyme in crude extracts Nostoc sp. MAC RNAn+1 + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.8 trimer alpha3, 3 * 91000, SDS-PAGE Nostoc sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.8 37
-
 assay at Nostoc sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.8 10.5
-
-
 Nostoc sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.8 9.5 11.5 14% of maximal activity at pH 9.5, 70% of maximal activity at pH 11.5 Nostoc sp.