Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Smith, J.C.; Eaton, M.A.W.
    Purification of polynucleotide phosphorylase by affinity chromatography and some properties of the purified enzymes (1974), Nucleic Acids Res., 1, 1763-1773.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.8 Escherichia coli
-
-
-
2.7.7.8 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.8 affinity chromatography Escherichia coli
2.7.7.8 polyethylene imine, Sepharose 4B, affinity chromatography on oligo(dT)-Sepharose Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.8 22.5
-
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.8 poly(A) + ADP
-
 Escherichia coli poly(A)+1 + phosphate
-
 r
2.7.7.8 poly(A) + ADP strong preference for ADP and poly(A) in phosphorolysis and polymerization reaction Geobacillus stearothermophilus poly(A)+1 + phosphate
-
 r
2.7.7.8 poly(A)+1 + phosphate
-
 Escherichia coli poly(A) + ADP
-
 r
2.7.7.8 poly(A)+1 + phosphate
-
 Geobacillus stearothermophilus poly(A) + ADP
-
 r
2.7.7.8 poly(I) + IDP
-
 Escherichia coli poly(I)+1 + phosphate
-
 ?
2.7.7.8 poly(I) + IDP
-
 Geobacillus stearothermophilus poly(I)+1 + phosphate phosphorolysis at 14% of activity with poly(A) ?
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of ADP Escherichia coli RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate polymerization of ADP Geobacillus stearothermophilus RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate strong preference for ADP Geobacillus stearothermophilus RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn+1 + phosphate phosphorolysis of poly(I) Escherichia coli RNAn + a nucleoside diphosphate
-
 r
2.7.7.8 RNAn+1 + phosphate phosphorolysis of poly(I) Geobacillus stearothermophilus RNAn + a nucleoside diphosphate
-
 r
2.7.7.8 RNAn+1 + phosphate strong preference for poly(A) Geobacillus stearothermophilus RNAn + a nucleoside diphosphate
-
 r
2.7.7.8 RNAn+1 + phosphate phosphorolysis of poly(A) Escherichia coli RNAn + a nucleoside diphosphate
-
 r
2.7.7.8 RNAn+1 + phosphate phosphorolysis of poly(A) Geobacillus stearothermophilus RNAn + a nucleoside diphosphate
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.8 8.2
-
 phosphorolysis of poly(A) Geobacillus stearothermophilus
2.7.7.8 10
-
 polymerization of ADP Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.8 7.2 9 phosphorolysis of poly(A), approx. 25% of maximal activity at pH 7.3 and pH 9.1, respectively Geobacillus stearothermophilus
2.7.7.8 8.4 10 polymerization of ADP, approx. 25% of maximal activity at pH 8.6 Geobacillus stearothermophilus