Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Khan, Z.A.; Fraenkel-Conrat, H.
    Purification and characterization of polynucleotide phosphorylase from cucumber (1985), Proc. Natl. Acad. Sci. USA, 82, 1311-1315.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.7.7.8 stable against several freezing and thawing cycles Cucumis sativus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.8 phosphate 0.5 mM, complete inhibition Cucumis sativus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.8 0.4
-
 UDP pH 8.0, 30°C Cucumis sativus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.8 50000
-
 3 * 50000, denaturing PAGE Cucumis sativus
2.7.7.8 150000
-
 glycerol density gradient centrifugation Cucumis sativus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.8 Cucumis sativus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.8 ammonium sulfate, polyethlene glycol 6000, DEAE-Sepharose, QAE-Sephadex, blue-Sepharose, glycerol gradient centrifugation Cucumis sativus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.8 cotyledon
-
 Cucumis sativus
-
2.7.7.8 leaf
-
 Cucumis sativus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.8 34.08
-
 incorporation of GDP Cucumis sativus

Storage Stability

EC Number Storage Stability Organism
2.7.7.8 -60°C, several months, no loss of activity Cucumis sativus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.8 RNAn + a nucleoside diphosphate activity in the absence of a primer, polymerization is stimulated by various polyribonucleotides or RNAs Cucumis sativus RNAn+1 + phosphate
-
 ?
2.7.7.8 RNAn + UDP
-
 Cucumis sativus RNAn+1 + phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.8 trimer 3 * 50000, denaturing PAGE Cucumis sativus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.8 30
-
-
 Cucumis sativus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.8 8.6
-
-
 Cucumis sativus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.8 7.5 9.3 approx. 60% of maximal activity at pH 7.5, approx. 70% of maximal activity at pH 9.3 Cucumis sativus