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Literature summary extracted from

  • Harry, K.; Sharma, N.; Fitt, P.S.
    Preparation and properties of highly-purified Vibrio costicola polynucleotide phosphorylase (1985), Biochim. Biophys. Acta, 828, 29-38.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.8 KCl salt is absolutely required for activity, maximal activity between 250 mM, inhibition above 1M Salinivibrio costicola
2.7.7.8 NaCl salt is absolutely required for activity, maximal activity at 250 mM, inhibition above 1 M Salinivibrio costicola
2.7.7.8 poly-L-lysine 0.25 mg/ml, 78fold activation in the presence of 10 mM NaCl, 5fold activation in the presence of 250 mM NaCl Salinivibrio costicola

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.8 Acridine orange inhibition of polymerization Salinivibrio costicola
2.7.7.8 NaCl stimulates polymerization maximally at 250 mM, inhibition above Salinivibrio costicola

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.8 Mg2+ divalent cation required, maximal activation at approx. 2 mM, Mg2+ is more effective than Mn2+ for polymerization, Mn2+ better activator in phosphorolytic reaction Salinivibrio costicola
2.7.7.8 Mn2+ divalent cation required, Mg2+ more effective than Mn2+ for polymerization, Mn2+ better activator in phosphorolytic reaction Salinivibrio costicola
2.7.7.8 Na+ NaCl stimulates polymerization maximally at250 mM, inhibition above Salinivibrio costicola

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.8 72000
-
 3 * 72000, SDS-PAGE in presence of 2-mercaptoethanol Salinivibrio costicola
2.7.7.8 220000
-
 native PAGE Salinivibrio costicola

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.8 Salinivibrio costicola
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.8 phosphocellulose, DEAE-Sephadex, Blue-Sepharose, Sephacryl s-300 Salinivibrio costicola

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.8 99.01
-
-
 Salinivibrio costicola

Storage Stability

EC Number Storage Stability Organism
2.7.7.8 -20°C, 100 mM NaCl, 0.1 mM EDTA, 0.1 mM dithiothreitol, 0.01 mM PMSF, 30% glycerol, 50 mM Tris-HCl, pH 8, at least 8 months, no loss of activity Salinivibrio costicola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.8 poly(C) + CDP
-
 Salinivibrio costicola poly(C)+1 + phosphate low poly(C) phosphorolysis activity r
2.7.7.8 poly(U)+ UDP
-
 Salinivibrio costicola poly(U)+1 + phosphate lower activity than with poly(A) r
2.7.7.8 RNAn + a nucleoside diphosphate
-
 Salinivibrio costicola RNAn+1 + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.8 trimer 3 * 72000, SDS-PAGE in presence of 2-mercaptoethanol Salinivibrio costicola

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.8 37
-
 assay at Salinivibrio costicola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.8 8 9 phosphorolysis Salinivibrio costicola
2.7.7.8 9.5
-
 or above, polymerization Salinivibrio costicola