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Literature summary extracted from

  • Soreq, H.; Littauer, U.Z.
    Purification and characterization of polynucleotide phosphorylase from Escherichia coli. Probe for the analysis of 3 sequences of RNA (1977), J. Biol. Chem., 252, 6885-6888.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.8 0.000166
-
 globin mRNA pH 8.0, 37°C, phosphorolysis of poly(A)tail from rabbit globin mRNA Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.8 230000
-
 ultracentrifugation, equilibrium sedimentaion analysis Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.8 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.8 ammonium sulfate, DEAE-cellulose, RNA-sepharose Escherichia coli

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.8 27.67
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.7.8 -20°C, 3 mg/ml enzyme concentration, 3 years, no loss of activity Escherichia coli
2.7.7.8 -20°C, dilute solution, at least 2 months, no loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.8 ADP + globin mRNAn
-
 Escherichia coli globin mRNAn+1 + phosphate
-
 ?
2.7.7.8 rabbit globin mRNAn+1 + phosphate only the poly(A) tail of the mRNA is removed Escherichia coli ADP + rabbit globin mRNAn
-
 ?
2.7.7.8 RNAn + a nucleoside diphosphate
-
 Escherichia coli RNAn+1 + phosphate
-
 r
2.7.7.8 RNAn+1 + phosphate processive phosphorolysis of the poly(A) tail of each globin mRNA chain Escherichia coli RNAn + a nucleoside diphosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.7.8 trimer SDS-PAGE Escherichia coli
2.7.7.8 trimer alpha3, 3 * 84000-95000, enzyme form A, ultrastructural observations Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.8 37
-
 assay at Escherichia coli

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.8 Escherichia coli isoelectric focusing
-
 6.1