Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Auer, T.; Landre, P.A.; Myers, T.W.
    Properties of the 5'->3' exonuclease/ribonuclease H activity of Thermus thermophilus DNA polymerase (1995), Biochemistry, 34, 4994-5002.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.7.7 biotechnology thermostable polymerase used in PCR Thermus thermophilus
2.7.7.7 molecular biology thermostable polymerase used in PCR Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.7 dimethyl sulfoxide 67% inhibition of RNAse H activity at 10% Thermus thermophilus
2.7.7.7 glycerol 33% inhibition of RNAse H activity at 10% Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.7 Mg2+ enzyme prefers Mn2+ over Mg2+ for RNase H activity Thermus thermophilus
2.7.7.7 Mn2+ enzyme prefers Mn2+ over Mg2+ for RNase H activity, optimal concentration: 1.5-2.5 mM Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn Thermus thermophilus
-
 diphosphate + DNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Thermus thermophilus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn enzyme also has RNAse H/exonuclease 5'--3' activity, enzyme prefers RNA/DNA substrate over DNA/DNA duplex Thermus thermophilus diphosphate + DNAn+1
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.7 70
-
-
 Thermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.7 70 75 optimal RNAse H activity at 70°C, sharp decline of activity above 75°C Thermus thermophilus