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Literature summary extracted from

  • Amblar, M.; Lopez, P.
    Purification and properties of the 5'-3' exonuclease D190->A mutant of DNA polymerase I from Streptococcus pneumoniae (1998), Eur. J. Biochem., 252, 124-132.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.7 wild-type and D10A mutant Streptococcus pneumoniae
2.7.7.7 wild-type and D190A mutant Streptococcus pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.7 D190A retains polymerase activity, reduced exonuclease activity, changes in dependency on metal activation of exonuclease activity Streptococcus pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.7 Mg2+ exonuclease acitivity shows equal efficiency with Mg2+ and Mn2+, mutant D190A shows preference for Mn2+ Streptococcus pneumoniae
2.7.7.7 Mn2+ exonuclease acitivity shows equal efficiency with Mg2+ and Mn2+, mutant D190A shows preference for Mn2+ Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn Streptococcus pneumoniae
-
 diphosphate + DNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Streptococcus pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7
-
 Streptococcus pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.7 4.7
-
 D190A mutant Streptococcus pneumoniae
2.7.7.7 9.84
-
 D10A mutant Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 ATP + primed M13
-
 Streptococcus pneumoniae ?
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Streptococcus pneumoniae diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn exonuclease 5'--3' activity, pol I Streptococcus pneumoniae diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + primed M13n
-
 Streptococcus pneumoniae diphosphate + primed M13n+1
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.7 0.000042
-
 primed M13 exonuclease activity, D190A mutant, pH 7.6, 37°C Streptococcus pneumoniae
2.7.7.7 0.011
-
 primed M13 exonuclease activity, wild-type, pH 7.6, 37°C Streptococcus pneumoniae
2.7.7.7 0.056
-
 ATP polymerase activity, D190A mutant, pH 7.6, 37°C Streptococcus pneumoniae