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Literature summary extracted from

  • Niranjanakumari, S.; Gopinathan, K.P.
    Isolation and characterization of DNA polymerase epsilon from the silk glands of Bombyx mori (1993), J. Biol. Chem., 268, 15557-15564.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.7 dimethyl sulfoxide inhibition of DNA polymerase epsilon, stimulation of DNA polymerase delta Bombyx mori

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.7 2-(p-n-Butylanilino)-2'-deoxyadenosine 5'-triphosphate inhibition of DNA polymerase alpha Bombyx mori
2.7.7.7 aphidicolin DNA polymerase epsilon, 80% inhibition at 0.002 mg/ml Bombyx mori
2.7.7.7 dimethyl sulfoxide inhibition of DNA polymerase epsilon, stimulation of DNA polymerase delta Bombyx mori
2.7.7.7 additional information no inhibition of DNA polymerase epsilon by 2-(p-n-butylanilino)-2'-deoxyadenosine 5'-triphosphate Bombyx mori
2.7.7.7 N2-(p-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate inhibition of DNA polymerase alpha Bombyx mori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.7 42000
-
 1 * 215000 + 1 * 42000, polymerase epsilon, denaturing PAGE Bombyx mori
2.7.7.7 215000
-
 1 * 215000 + 1 * 42000, polymerase epsilon, denaturing PAGE Bombyx mori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn Bombyx mori
-
 diphosphate + DNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Bombyx mori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7 DNA polymerase alpha, delta and epsilon Bombyx mori

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.7 larva
-
 Bombyx mori
-
2.7.7.7 silk gland
-
 Bombyx mori
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.7 0.015
-
 polymerase epsilon, exonuclease activity Bombyx mori
2.7.7.7 0.031
-
 polymerase epsilon, polymerase activity Bombyx mori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Bombyx mori diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn exonuclease 3'--5' activity, pol epsilon Bombyx mori diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn template specificity of DNA polymerase epsilon Bombyx mori diphosphate + DNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.7 dimer 1 * 215000 + 1 * 42000, polymerase epsilon, denaturing PAGE Bombyx mori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.7 6.5
-
-
 Bombyx mori