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Literature summary extracted from

  • Burrows, J.A.; Goward, C.R.
    Purification and properties of DNA polymerase from Bacillus caldotenax (1992), Biochem. J., 287, 971-977.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.7 aphidicolin 50% inhibition at 0.05 mM [Bacillus] caldotenax
2.7.7.7 Ara-CTP 50% inhibition at 0.05 mM [Bacillus] caldotenax
2.7.7.7 Dideoxythymidine triphosphate 50% inhibition at 0.25 mM [Bacillus] caldotenax

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.7 Co2+ can partially replace Mg2+ in activation, optimal concentration: 2.5 mM [Bacillus] caldotenax
2.7.7.7 K+ stimulates at 100 mM [Bacillus] caldotenax
2.7.7.7 Mg2+ stimulates at 70 mM [Bacillus] caldotenax
2.7.7.7 Mn2+ can partially replace Mg2+ in activation [Bacillus] caldotenax

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.7 94000
-
 1 * 94000, SDS-PAGE [Bacillus] caldotenax
2.7.7.7 110000 120000 HPLC gel filtration, non-denaturing PAGE [Bacillus] caldotenax

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn [Bacillus] caldotenax
-
 diphosphate + DNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 [Bacillus] caldotenax
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7
-
 [Bacillus] caldotenax

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.7 additional information
-
-
 [Bacillus] caldotenax

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 [Bacillus] caldotenax diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn template specificity [Bacillus] caldotenax diphosphate + DNAn+1
-
 ?
2.7.7.7 dNTP + DNAn
-
 [Bacillus] caldotenax diphosphate + DNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.7 monomer 1 * 94000, SDS-PAGE [Bacillus] caldotenax

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.7 70
-
-
 [Bacillus] caldotenax

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.7 37 55 37°C: 15% of activity maximum, 55°C: 65% of activity maximum [Bacillus] caldotenax

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.7 70
-
 t1/2: 1.5 h without glycerol, 20 h in presence of glycerol [Bacillus] caldotenax

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.7 1
-
 dNTP
-
 [Bacillus] caldotenax
2.7.7.7 1.05
-
 deoxynucleoside triphosphate equimolar mixture of all nucleotides, pH 7.5, 70°C [Bacillus] caldotenax

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.7 7 8
-
 [Bacillus] caldotenax

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.7 [Bacillus] caldotenax PAGE, pH range 3-9, pH 7.5, 70°C
-
 4.9