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Literature summary extracted from

  • Gass, K.B.; Cozzarelli, N.R.
    Bacillus subtilis DNA polymerases (1974), Methods Enzymol., 29, 27-38.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.7.7 unstable in solutions with low ionic strength, glycerol and bovine serum albumin stabilize Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.7 4-chloromercuribenzoic acid
-
 Bacillus subtilis
2.7.7.7 Ara-CTP polymerase II and III Bacillus subtilis
2.7.7.7 hydroxyphenylazouracil inhibits only polymerase III Bacillus subtilis
2.7.7.7 KCl
-
 Bacillus subtilis
2.7.7.7 additional information no inhibition of polymerase I by ara-CTP Bacillus subtilis
2.7.7.7 N-ethylmaleimide polymerase I and II; polymerase III Bacillus subtilis
2.7.7.7 single-stranded DNA
-
 Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn Bacillus subtilis polymerase I plays a role in repair of chromosomal damage diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn Bacillus subtilis polymerase III is necessary for DNA replication diphosphate + DNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7 polymerase I, II and III Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.7 0.042
-
 polymerase III Bacillus subtilis
2.7.7.7 0.4
-
 polymerase II Bacillus subtilis

Storage Stability

EC Number Storage Stability Organism
2.7.7.7 -60°C, stable for 60 days Bacillus subtilis
2.7.7.7 0°C, 3 weeks stable in 0.5 M potassium phosphate, 2 M ammonium sulfate or 4 M NaCl Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn template specificity polymerase I, II and III Bacillus subtilis diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn polymerase I plays a role in repair of chromosomal damage Bacillus subtilis diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn polymerase III is necessary for DNA replication Bacillus subtilis diphosphate + DNAn+1
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.7 additional information
-
 heat stability of polymerase I, II and III Bacillus subtilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.7 0.001
-
 hydroxyphenylazouracil pH 7.5, 37°C Bacillus subtilis