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Literature summary extracted from

  • Lehman, R.
    T-phage polymerases (1981), The Enzymes,3rd Ed. (Boyer,P. D. ,ed. ), 14, 51-65.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.7 thiol stimulates Escherichia phage T7
2.7.7.7 thiol required Tequintavirus T5

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.7 N-ethylmaleimide great sensitivity of phage-induced enzyme, relative insensitivity of pol I Escherichia phage T7
2.7.7.7 N-ethylmaleimide great sensitivity of phage-induced enzyme, relative insensitivity of pol I Tequatrovirus T4
2.7.7.7 N-ethylmaleimide great sensitivity of phage-induced enzyme, relative insensitivity of pol I Tequintavirus T5

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.7 Mg2+ divalent cation required, Mg2+ or Mn2+ Tequatrovirus T4
2.7.7.7 Mg2+ divalent cation required, Mg2+ or Mn2+ Escherichia phage T7
2.7.7.7 Mg2+ divalent cation required, Mg2+ or Mn2+ Tequintavirus T5
2.7.7.7 Mg2+ optimal concentration: 6 mM Tequatrovirus T4
2.7.7.7 Mn2+ optimal concentration: 0.1 mM Tequatrovirus T4
2.7.7.7 Mn2+ 25% of the activity with Mg2+ Tequatrovirus T4
2.7.7.7 Mn2+ divalent cation required, Mn2+ or Mg2+ Tequatrovirus T4
2.7.7.7 Mn2+ divalent cation required, Mn2+ or Mg2+ Escherichia phage T7
2.7.7.7 Mn2+ divalent cation required, Mn2+ or Mg2+ Tequintavirus T5
2.7.7.7 Na+ 0.2 M, stimulates phage T5-induced enzyme Tequintavirus T5
2.7.7.7 NH4+ 0.2 M, stimulates phage T5-induced enzyme Tequintavirus T5

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.7 12000
-
 1 * 12000 thioredoxin + 1 * 84000 Escherichia phage T7
2.7.7.7 84000
-
 1 * 12000 thioredoxin + 1 * 84000 Escherichia phage T7
2.7.7.7 96000
-
 equilibrium sedimentation Escherichia phage T7
2.7.7.7 96000
-
 equilibrium sedimentation Tequintavirus T5
2.7.7.7 114000
-
-
 Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn Escherichia phage T7
-
 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn Tequintavirus T5
-
 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn Tequatrovirus T4 phage T4 DNA polymerase is essential for initiation and maintenance of viral DNA replication diphosphate + DNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Escherichia phage T7
-
 Escherichia coli infected with
-
2.7.7.7 Tequatrovirus T4
-
 Escherichia coli infected with
-
2.7.7.7 Tequintavirus T5
-
 Escherichia coli infected with
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7 Escherichia coli infected with Tequatrovirus T4
2.7.7.7 Escherichia coli infected with Escherichia phage T7
2.7.7.7 Escherichia coli infected with Tequintavirus T5

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Escherichia phage T7 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Tequintavirus T5 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn exonuclease 3'--5' activity and 5'--3' activity, phage T7-induced DNA polymerase Escherichia phage T7 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn nicked duplex is no substrate of phage T4-induced DNA polymerase Tequatrovirus T4 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn nicked duplex is no substrate of phage T4-induced DNA polymerase Escherichia phage T7 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn exonuclease 3'--5' and 5'--3' activity activity, phage T5-induced DNA polymerase Tequintavirus T5 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn phage T5-induced DNA polymerase Tequintavirus T5 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA Tequatrovirus T4 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA Escherichia phage T7 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA Tequintavirus T5 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn exonuclease 3'--5', phage T4-induced DNA polymerase Tequatrovirus T4 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn primed single strands Tequatrovirus T4 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn phage T4 DNA polymerase is essential for initiation and maintenance of viral DNA replication Tequatrovirus T4 diphosphate + DNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.7 dimer 1 * 12000 thioredoxin + 1 * 84000 Escherichia phage T7
2.7.7.7 monomer 1 * 114000 Tequatrovirus T4
2.7.7.7 monomer 1 * 96000 Tequintavirus T5

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.7 7.6 7.8
-
 Escherichia phage T7
2.7.7.7 8.5 9
-
 Tequintavirus T5

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.7 7.5 9.7 about 50% of activity maximum at pH 7.5 and pH 9.7 Tequatrovirus T4