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Literature summary extracted from

  • Kornberg, T.; Kornberg, A.
    Bacterial DNA polymerases (1974), The Enzymes,3rd Ed. (Boyer,P. D. ,ed. ), 10, 119-144.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.7 Ara-CTP no inhibition of pol I and III Bacillus subtilis
2.7.7.7 Ara-CTP pol II Escherichia coli
2.7.7.7 hydroxyphenylazouracil inhibits only polymerase III Bacillus subtilis
2.7.7.7 additional information
-
 Bacillus subtilis
2.7.7.7 additional information
-
 Bacteria
2.7.7.7 additional information pol II and III not inhibited by pol I antiserum Escherichia coli
2.7.7.7 additional information
-
 Micrococcus luteus
2.7.7.7 SH-blocking agents pol II and III Bacillus subtilis
2.7.7.7 SH-blocking agents pol II and III Bacteria
2.7.7.7 SH-blocking agents pol II and III Escherichia coli
2.7.7.7 SH-blocking agents pol II and III Micrococcus luteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.7 109000
-
 pol I Escherichia coli
2.7.7.7 110000
-
 polymerase I Micrococcus luteus
2.7.7.7 115000
-
 polymerase I Bacillus subtilis
2.7.7.7 160000
-
 pol III Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn Bacteria
-
 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn Micrococcus luteus
-
 diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn Bacillus subtilis physiological role of pol I and pol III diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn Escherichia coli physiological role of pol I, II and pol III diphosphate + DNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Bacillus subtilis
-
-
-
2.7.7.7 Bacteria
-
-
-
2.7.7.7 Escherichia coli
-
 pol II
-
2.7.7.7 Escherichia coli
-
 pol I
-
2.7.7.7 Escherichia coli
-
 pol III
-
2.7.7.7 Micrococcus luteus
-
 synonym: Micrococcus lysodeikticus
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.7 a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 mechanism Bacteria
a
2.7.7.7 a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 mechanism Bacillus subtilis
a
2.7.7.7 a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 mechanism Escherichia coli
a
2.7.7.7 a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 mechanism Micrococcus luteus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Bacteria diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Micrococcus luteus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn exonuclease 3'--5' activity, pol III Escherichia coli diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn no exonuclease 5'--3' activity: pol II Bacteria diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn no exonuclease 5'--3' activity: pol II Bacillus subtilis diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn no exonuclease 5'--3' activity: pol II Escherichia coli diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn no exonuclease 5'--3' activity: pol II Micrococcus luteus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn exonuclease 3'--5' activity, pol II Escherichia coli diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn can initiate polymer synthesis de novo, pol I Bacillus subtilis diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn can initiate polymer synthesis de novo, pol I Escherichia coli diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn can initiate polymer synthesis de novo, pol I Micrococcus luteus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn single strands, pol I, but not pol II and III Escherichia coli diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn single strands, pol I, but not pol II and III Micrococcus luteus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn single-stranded 5'-ends greater than 100 nucleotides, pol I, but not pol II and pol III Escherichia coli diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn single-stranded 5'-ends greater than 100 nucleotides, pol I, but not pol II and pol III Micrococcus luteus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA Bacteria diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA Bacillus subtilis diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA Escherichia coli diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA Micrococcus luteus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn exonuclease 3'--5' activity, pol I Escherichia coli diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn exonuclease 3'--5' activity, pol I Micrococcus luteus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn nicked duplex is no substrate of polymerase I Bacillus subtilis diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn nicked duplex is no substrate of polymerase I Micrococcus luteus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn nicked duplex is no substrate of pol II and III of E. coli Escherichia coli diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn nicked duplex, as poly d(A-T), pol I Bacteria diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn nicked duplex, as poly d(A-T), pol I Bacillus subtilis diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn nicked duplex, as poly d(A-T), pol I Escherichia coli diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn nicked duplex, as poly d(A-T), pol I Micrococcus luteus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn can not initiate polymer synthesis de novo: pol II and III Escherichia coli diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn exonuclease 5'--3' activity, pol I Escherichia coli diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn exonuclease 5'--3' activity, pol I Micrococcus luteus diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn physiological role of pol I and pol III Bacillus subtilis diphosphate + DNAn+1
-
 ?
2.7.7.7 deoxynucleoside triphosphate + DNAn physiological role of pol I, II and pol III Escherichia coli diphosphate + DNAn+1
-
 ?
2.7.7.7 nucleotide + DNAn
-
 Escherichia coli ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.7 37
-
 pol I and II stable, pol III not Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.7 4.17
-
 Nucleotide pol II Escherichia coli
2.7.7.7 16.7
-
 Nucleotide pol I Escherichia coli
2.7.7.7 250
-
 Nucleotide pol III Escherichia coli