EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.6 | (NH4)2SO4 | - |
Pisum sativum | |
2.7.7.6 | heparin | - |
Pisum sativum | |
2.7.7.6 | Tagetitoxin | 50% inhibition at 0.0001 mM. Complete inhibition at 0.01 mM | Pisum sativum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.6 | 0.1 | - |
GTP | pH 7.9, 23°C | Pisum sativum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.7.6 | chloroplast | - |
Pisum sativum | 9507 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.6 | KCl | maximal activity at 2 mM KCl | Pisum sativum | |
2.7.7.6 | Mg2+ | maximal activity at 15 mM MgCl2 or MnCl2 | Pisum sativum | |
2.7.7.6 | Mn2+ | maximal activity at 15 mM MgCl2 or MnCl2 | Pisum sativum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.7.6 | 35000 | - |
x * 120000 + x * 110000 + x * 95000 + x * 84000 + x * 81000 + x * 75000 + x * 54000 + x * 51000 + x * 42000 + x * 35000, the 110000 Da polypeptide binds nucleoside triphosphates, the 42000 Da polypeptide cross-reacts with antiserum raised to the plastid endoded rpoA gene product, SDS-PAGE | Pisum sativum |
2.7.7.6 | 42000 | - |
x * 120000 + x * 110000 + x * 95000 + x * 84000 + x * 81000 + x * 75000 + x * 54000 + x * 51000 + x * 42000 + x * 35000, the 110000 Da polypeptide binds nucleoside triphosphates, the 42000 Da polypeptide cross-reacts with antiserum raised to the plastid endoded rpoA gene product, SDS-PAGE | Pisum sativum |
2.7.7.6 | 51000 | - |
x * 120000 + x * 110000 + x * 95000 + x * 84000 + x * 81000 + x * 75000 + x * 54000 + x * 51000 + x * 42000 + x * 35000, the 110000 Da polypeptide binds nucleoside triphosphates, the 42000 Da polypeptide cross-reacts with antiserum raised to the plastid endoded rpoA gene product, SDS-PAGE | Pisum sativum |
2.7.7.6 | 54000 | - |
x * 120000 + x * 110000 + x * 95000 + x * 84000 + x * 81000 + x * 75000 + x * 54000 + x * 51000 + x * 42000 + x * 35000, the 110000 Da polypeptide binds nucleoside triphosphates, the 42000 Da polypeptide cross-reacts with antiserum raised to the plastid endoded rpoA gene product, SDS-PAGE | Pisum sativum |
2.7.7.6 | 75000 | - |
x * 120000 + x * 110000 + x * 95000 + x * 84000 + x * 81000 + x * 75000 + x * 54000 + x * 51000 + x * 42000 + x * 35000, the 110000 Da polypeptide binds nucleoside triphosphates, the 42000 Da polypeptide cross-reacts with antiserum raised to the plastid endoded rpoA gene product, SDS-PAGE | Pisum sativum |
2.7.7.6 | 81000 | - |
x * 120000 + x * 110000 + x * 95000 + x * 84000 + x * 81000 + x * 75000 + x * 54000 + x * 51000 + x * 42000 + x * 35000, the 110000 Da polypeptide binds nucleoside triphosphates, the 42000 Da polypeptide cross-reacts with antiserum raised to the plastid endoded rpoA gene product, SDS-PAGE | Pisum sativum |
2.7.7.6 | 84000 | - |
x * 120000 + x * 110000 + x * 95000 + x * 84000 + x * 81000 + x * 75000 + x * 54000 + x * 51000 + x * 42000 + x * 35000, the 110000 Da polypeptide binds nucleoside triphosphates, the 42000 Da polypeptide cross-reacts with antiserum raised to the plastid endoded rpoA gene product, SDS-PAGE | Pisum sativum |
2.7.7.6 | 95000 | - |
x * 120000 + x * 110000 + x * 95000 + x * 84000 + x * 81000 + x * 75000 + x * 54000 + x * 51000 + x * 42000 + x * 35000, the 110000 Da polypeptide binds nucleoside triphosphates, the 42000 Da polypeptide cross-reacts with antiserum raised to the plastid endoded rpoA gene product, SDS-PAGE | Pisum sativum |
2.7.7.6 | 110000 | - |
x * 120000 + x * 110000 + x * 95000 + x * 84000 + x * 81000 + x * 75000 + x * 54000 + x * 51000 + x * 42000 + x * 35000, the 110000 Da polypeptide binds nucleoside triphosphates, the 42000 Da polypeptide cross-reacts with antiserum raised to the plastid endoded rpoA gene product, SDS-PAGE | Pisum sativum |
2.7.7.6 | 120000 | - |
x * 120000 + x * 110000 + x * 95000 + x * 84000 + x * 81000 + x * 75000 + x * 54000 + x * 51000 + x * 42000 + x * 35000, the 110000 Da polypeptide binds nucleoside triphosphates, the 42000 Da polypeptide cross-reacts with antiserum raised to the plastid endoded rpoA gene product, SDS-PAGE | Pisum sativum |
2.7.7.6 | 620000 | - |
gel filtration | Pisum sativum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.6 | Pisum sativum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.6 | - |
Pisum sativum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.7.6 | leaf | - |
Pisum sativum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.7.6 | 0.006 | - |
- |
Pisum sativum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.6 | GTP + RNAn | - |
Pisum sativum | diphosphate + RNAn+1 | - |
? | |
2.7.7.6 | nucleoside triphosphate + RNAn | the enzyme requires DNA as template | Pisum sativum | diphosphate + RNAn+1 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.7.6 | ? | x * 120000 + x * 110000 + x * 95000 + x * 84000 + x * 81000 + x * 75000 + x * 54000 + x * 51000 + x * 42000 + x * 35000, the 110000 Da polypeptide binds nucleoside triphosphates, the 42000 Da polypeptide cross-reacts with antiserum raised to the plastid endoded rpoA gene product, SDS-PAGE | Pisum sativum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.6 | 40 | - |
- |
Pisum sativum |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.6 | 30 | 50 | 30°C: about 50% of maximal activity, 50°C: about 20% of maximal activity | Pisum sativum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.6 | 7.9 | - |
- |
Pisum sativum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.6 | 7 | 8.7 | pH 7.0: about 75% of maximal activity, pH 8.7: about 75% of maximal activity | Pisum sativum |