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Literature summary extracted from

  • Azuma, Y.; Yamagishi, M.; Ishihama, A.
    Subunits of the Schizosaccharomyces pombe RNA polymerase II: enzyme purification and structure of the subunit 3 gene (1993), Nucleic Acids Res., 21, 3749-3754.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.6 subunit 3 gene, rpb3 Schizosaccharomyces pombe

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.6 13000
-
x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe
2.7.7.6 13500
-
x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe
2.7.7.6 15000
-
x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe
2.7.7.6 16000
-
x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe
2.7.7.6 20000
-
x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe
2.7.7.6 25000
-
x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe
2.7.7.6 33000
-
x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe
2.7.7.6 34000
-
x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe
2.7.7.6 40000
-
x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe
2.7.7.6 150000
-
x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe
2.7.7.6 210000
-
x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.6 Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.6 RNA polymerase II Schizosaccharomyces pombe

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.6 0.6
-
-
Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.6 nucleoside triphosphate + RNAn the enzyme requires DNA as template Schizosaccharomyces pombe diphosphate + RNAn+1
-
?

Subunits

EC Number Subunits Comment Organism
2.7.7.6 ? x * 210000 + x * 150000 + x * 40000 + x * 34000 + x * 33000 + x * 25000 + x * 20000 + x * 16000 + x * 15000 + x * 13500 + x * 13000, the enzyme contains more than eleven polypeptides, SDS-PAGE Schizosaccharomyces pombe