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Literature summary extracted from

  • Pich, A.; Baghl, H.
    Purification and characterization of the DNA-dependent RNA polymerase from Clostridium acetobutylicum (1991), J. Bacteriol., 173, 2120-2124.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.7.7.6 40% glycerol is absolutely required for stability Clostridium acetobutylicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.6 rifampicin 0.1 mg/ml, complete inhibition Clostridium acetobutylicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.6 Co2+ can replace Mg2+ in activation, 10% of the activity observed with Mg2+ Clostridium acetobutylicum
2.7.7.6 Mg2+ highest activity is obtained with 20 mM Mg2+ on poly(dA-dT) DNA or Clostridium acetobutylicum DNA as template, with calf thymus DNA as template maximal activity is achieved with 10 mM Mg2+ Clostridium acetobutylicum
2.7.7.6 Mn2+ 3 mM Mn2+ results in comparable activity with poly(dA-dT) DNA but reduced activity on calf thymus DNA or Clostridium acetobutylicum DNA and Clostridium acetobutylicum DNA as substrated, compared to Mg2+ activition Clostridium acetobutylicum

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.6 Clostridium acetobutylicum
-
 DSM 1731
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.6
-
 Clostridium acetobutylicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.6 0.0527
-
-
 Clostridium acetobutylicum

Storage Stability

EC Number Storage Stability Organism
2.7.7.6 -20°C, 60 mM Tris-HCl buffer, pH 7.8, 20 mM NH4Cl, 1 mM EDTA, 10 mM 2-mercaptoethanol, 40% glycerol, stable for several months Clostridium acetobutylicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.6 nucleoside triphosphate + RNAn absolutely dependent on the presence of a double-stranded or single-stranded DNA template Clostridium acetobutylicum diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn with poly(dA-dT) DNA as template Clostridium acetobutylicum diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn the enzyme requires DNA as template Clostridium acetobutylicum diphosphate + RNAn+1
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.6 40 45 with poly(dA-dT) DNA or Clostridium acetobutylicum DNA as template Clostridium acetobutylicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.6 10
-
 poly(dA-dT) DNA as a template Clostridium acetobutylicum