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Literature summary extracted from

  • Borbely, G.; Schneider, G.J.
    Cyanobacterial DNA-dependent RNA polymerase (1988), Methods Enzymol., 167, 592-599.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.7.6 enzyme shows an extreme instability in the presence of salts Microchaete diplosiphon

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.6 Mg2+ required, maximal activity at 10-20 mM Synechococcus elongatus PCC 7942 = FACHB-805
2.7.7.6 Mg2+ required, maximal activity at 10-20 mM Anabaena sp.
2.7.7.6 Mg2+ only limited ability to replace Mn2+ Anabaena cylindrica
2.7.7.6 Mg2+ 30 mM Mg2+ or 5 mM Mn2+ work equally well for maximal activity Microchaete diplosiphon
2.7.7.6 Mn2+ can partially replace Mg2+ in activation Synechococcus elongatus PCC 7942 = FACHB-805
2.7.7.6 Mn2+ can partially replace Mg2+ in activation Anabaena sp.
2.7.7.6 Mn2+ 30 mM Mg2+ or 5 mM Mn2+ work equally well for maximal activity Microchaete diplosiphon
2.7.7.6 Mn2+ 2-4 mM required for optimal activity Anabaena cylindrica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.6 30000
-
 x * 185000 + x * 125000 + x * 47000 + x * 30000 + x * 93000, SDS-PAGE Anabaena cylindrica
2.7.7.6 38000
-
 x * 190000 + x * 145000 + x * 72000 + x * 38000, SDS-PAGE Synechococcus elongatus PCC 7942 = FACHB-805
2.7.7.6 41000
-
 x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Synechococcus elongatus PCC 7942 = FACHB-805
2.7.7.6 41000
-
 x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Anabaena sp.
2.7.7.6 41000
-
 x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Anabaena cylindrica
2.7.7.6 41000
-
 x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Microchaete diplosiphon
2.7.7.6 41000
-
 x * 161000 + x * 134000 + x * 72000 + x * 41000 + x * 91000, SDS-PAGE Microchaete diplosiphon
2.7.7.6 47000
-
 x * 185000 + x * 125000 + x * 47000 + x * 30000 + x * 93000, SDS-PAGE Anabaena cylindrica
2.7.7.6 52000
-
 x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Synechococcus elongatus PCC 7942 = FACHB-805
2.7.7.6 52000
-
 x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Anabaena sp.
2.7.7.6 52000
-
 x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Anabaena cylindrica
2.7.7.6 52000
-
 x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Microchaete diplosiphon
2.7.7.6 66000
-
 x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Synechococcus elongatus PCC 7942 = FACHB-805
2.7.7.6 66000
-
 x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Anabaena sp.
2.7.7.6 66000
-
 x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Anabaena cylindrica
2.7.7.6 66000
-
 x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Microchaete diplosiphon
2.7.7.6 72000
-
 x * 161000 + x * 134000 + x * 72000 + x * 41000 + x * 91000, SDS-PAGE Microchaete diplosiphon
2.7.7.6 72000
-
 x * 190000 + x * 145000 + x * 72000 + x * 38000, SDS-PAGE Synechococcus elongatus PCC 7942 = FACHB-805
2.7.7.6 91000
-
 x * 161000 + x * 134000 + x * 72000 + x * 41000 + x * 91000, SDS-PAGE Microchaete diplosiphon
2.7.7.6 93000
-
 x * 185000 + x * 125000 + x * 47000 + x * 30000 + x * 93000, SDS-PAGE Anabaena cylindrica
2.7.7.6 124000
-
 x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Synechococcus elongatus PCC 7942 = FACHB-805
2.7.7.6 124000
-
 x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Anabaena sp.
2.7.7.6 124000
-
 x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Anabaena cylindrica
2.7.7.6 124000
-
 x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Microchaete diplosiphon
2.7.7.6 125000
-
 x * 185000 + x * 125000 + x * 47000 + x * 30000 + x * 93000, SDS-PAGE Anabaena cylindrica
2.7.7.6 134000
-
 x * 161000 + x * 134000 + x * 72000 + x * 41000 + x * 91000, SDS-PAGE Microchaete diplosiphon
2.7.7.6 145000
-
 x * 190000 + x * 145000 + x * 72000 + x * 38000, SDS-PAGE Synechococcus elongatus PCC 7942 = FACHB-805
2.7.7.6 161000
-
 x * 161000 + x * 134000 + x * 72000 + x * 41000 + x * 91000, SDS-PAGE Microchaete diplosiphon
2.7.7.6 171000
-
 x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Synechococcus elongatus PCC 7942 = FACHB-805
2.7.7.6 171000
-
 x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Anabaena sp.
2.7.7.6 171000
-
 x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Anabaena cylindrica
2.7.7.6 171000
-
 x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Microchaete diplosiphon
2.7.7.6 185000
-
 x * 185000 + x * 125000 + x * 47000 + x * 30000 + x * 93000, SDS-PAGE Anabaena cylindrica
2.7.7.6 190000
-
 x * 190000 + x * 145000 + x * 72000 + x * 38000, SDS-PAGE Synechococcus elongatus PCC 7942 = FACHB-805

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.6 Anabaena cylindrica
-
-
-
2.7.7.6 Anabaena sp.
-
 PCC 7120
-
2.7.7.6 Microchaete diplosiphon
-
-
-
2.7.7.6 Synechococcus elongatus PCC 7942 = FACHB-805
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.6 PCC 7120 Anabaena sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.6 0.0156
-
-
 Anabaena sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.6 nucleoside triphosphate + RNAn the enzyme requires DNA as template Synechococcus elongatus PCC 7942 = FACHB-805 diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn the enzyme requires DNA as template Anabaena sp. diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn the enzyme requires DNA as template Anabaena cylindrica diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn the enzyme requires DNA as template Microchaete diplosiphon diphosphate + RNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.6 ? x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Synechococcus elongatus PCC 7942 = FACHB-805
2.7.7.6 ? x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Anabaena sp.
2.7.7.6 ? x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Anabaena cylindrica
2.7.7.6 ? x * 171000 + x * 124000 + x * 66000 + x * 52000 + x * 41000, SDS-PAGE Microchaete diplosiphon
2.7.7.6 ? x * 161000 + x * 134000 + x * 72000 + x * 41000 + x * 91000, SDS-PAGE Microchaete diplosiphon
2.7.7.6 ? x * 190000 + x * 145000 + x * 72000 + x * 38000, SDS-PAGE Synechococcus elongatus PCC 7942 = FACHB-805
2.7.7.6 ? x * 185000 + x * 125000 + x * 47000 + x * 30000 + x * 93000, SDS-PAGE Anabaena cylindrica