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Literature summary extracted from

  • Zhou, Z.; Deutscher, M.P.
    An essential function for the phosphate-dependent exoribonucleases RNase PH and polynucleotide phosphorylase (1997), J. Bacteriol., 179, 4391-4395.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.56 additional information enzyme mutant deficient in tRNA nucleotidyltransferase and polynucleotide phosphorylase activity grows slowly at 37°C, shows a dramatically reduced tRNATyrsu3+ suppressor activity, displays reversible cold-sensitivity, and performs normal tRNA synthesis, ribosome structure and function are severely affected, particularly at lower temperatures of 31°C and below Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.8 RNAn + a nucleoside diphosphate Escherichia coli PNPase exonuclease activity plays an essential role in tRNA, mRNA and ribosome metabolism RNAn+1 + phosphate
-
 ?
2.7.7.56 additional information Escherichia coli phosphate-dependent tRNA nucleotidyltransferase and polynucleotide phosphorylase activity play an essential role that affects ribosome metabolism, this function cannot be taken over by any of the hydrolytic exonucleases present in the cell ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.8 Escherichia coli
-
-
-
2.7.7.56 Escherichia coli
-
 enzyme mutant deficient in tRNA nucleotidyltransferase and polynucleotide phosphorylase activity
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.8 RNAn+1 + phosphate = RNAn + a nucleoside diphosphate PNPase exhibits 3'-to-5' exonucleolytic activity Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.8 RNAn + a nucleoside diphosphate PNPase exonuclease activity plays an essential role in tRNA, mRNA and ribosome metabolism Escherichia coli RNAn+1 + phosphate
-
 ?
2.7.7.56 additional information phosphate-dependent tRNA nucleotidyltransferase and polynucleotide phosphorylase activity play an essential role that affects ribosome metabolism, this function cannot be taken over by any of the hydrolytic exonucleases present in the cell Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.56 More enzyme mutant deficient in tRNA nucleotidyltransferase and polynucleotide phosphorylase activity shows differing ribosome structure, the 50S subunit is reduced, the 23S subunit is degraded Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.56 additional information
-
 enzyme mutant deficient in tRNA nucleotidyltransferase and polynucleotide phosphorylase activity displays reversible cold-sensitivity, ribosome structure and function are severely affected, particularly at lower temperatures of 31°C and below Escherichia coli