BRENDA - Enzyme Database

An alleged yeast polyphosphate kinase is actually diadenosine-5', 5''-P1,P4-tetraphosphate alpha,beta-phosphorylase

Booth, J.W.; Guidotti, G.; J. Biol. Chem. 270, 19377-19382 (1995)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.53
AMP
ADP/phosphate-exchange
Saccharomyces cerevisiae
2.7.7.53
Mg2+
inhibitory in presence of phosphate for ADP/phosphate exchange reaction
Saccharomyces cerevisiae
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate
ADP/phosphate-exchange
Saccharomyces cerevisiae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.53
Mg2+
inhibitory in presence of phosphate for ADP/phosphate exchange reaction
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.53
40000
-
1 * 40000, SDS-PAGE
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.53
Saccharomyces cerevisiae
-
2 isoforms APA1 and APA2
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.53
ADP + phosphate
90% activity due to isoform I
643514
Saccharomyces cerevisiae
phosphate + ADP
-
643514
Saccharomyces cerevisiae
r
2.7.7.53
ADP + phosphate
ADP/phosphate exchange reaction
643514
Saccharomyces cerevisiae
phosphate + ADP
-
643514
Saccharomyces cerevisiae
r
2.7.7.53
NDP + phosphate
enzyme performs the exchange reaction also with polyphosphates
643514
Saccharomyces cerevisiae
phosphate + NDP
-
643514
Saccharomyces cerevisiae
r
2.7.7.53
NDP + phosphate
90% activity due to isoform I
643514
Saccharomyces cerevisiae
phosphate + NDP
-
643514
Saccharomyces cerevisiae
r
2.7.7.53
NDP + polyphosphate
exchange reaction
643514
Saccharomyces cerevisiae
polyphosphate + NDP
-
643514
Saccharomyces cerevisiae
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate
irreversible formation of reaction intermediate AMP-enzyme, unstable
643514
Saccharomyces cerevisiae
?
-
-
-
ir
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
AMP-enzyme intermediate
643514
Saccharomyces cerevisiae
ADP + ATP
-
643514
Saccharomyces cerevisiae
r
Subunits
EC Number
Subunits
Commentary
Organism
2.7.7.53
monomer
1 * 40000, SDS-PAGE
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.53
AMP
ADP/phosphate-exchange
Saccharomyces cerevisiae
2.7.7.53
Mg2+
inhibitory in presence of phosphate for ADP/phosphate exchange reaction
Saccharomyces cerevisiae
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate
ADP/phosphate-exchange
Saccharomyces cerevisiae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.53
Mg2+
inhibitory in presence of phosphate for ADP/phosphate exchange reaction
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.53
40000
-
1 * 40000, SDS-PAGE
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.53
ADP + phosphate
90% activity due to isoform I
643514
Saccharomyces cerevisiae
phosphate + ADP
-
643514
Saccharomyces cerevisiae
r
2.7.7.53
ADP + phosphate
ADP/phosphate exchange reaction
643514
Saccharomyces cerevisiae
phosphate + ADP
-
643514
Saccharomyces cerevisiae
r
2.7.7.53
NDP + phosphate
enzyme performs the exchange reaction also with polyphosphates
643514
Saccharomyces cerevisiae
phosphate + NDP
-
643514
Saccharomyces cerevisiae
r
2.7.7.53
NDP + phosphate
90% activity due to isoform I
643514
Saccharomyces cerevisiae
phosphate + NDP
-
643514
Saccharomyces cerevisiae
r
2.7.7.53
NDP + polyphosphate
exchange reaction
643514
Saccharomyces cerevisiae
polyphosphate + NDP
-
643514
Saccharomyces cerevisiae
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate
irreversible formation of reaction intermediate AMP-enzyme, unstable
643514
Saccharomyces cerevisiae
?
-
-
-
ir
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
AMP-enzyme intermediate
643514
Saccharomyces cerevisiae
ADP + ATP
-
643514
Saccharomyces cerevisiae
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.7.53
monomer
1 * 40000, SDS-PAGE
Saccharomyces cerevisiae