BRENDA - Enzyme Database

Specific phosphorylase from Euglena gracilis splits diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) and diadenosine 5',5'''-P1,P3-tetraphosphate (Ap3A)

Guranowski, A.; Starzynska, E.; Wasternack, C.; Int. J. Biochem. 20, 449-455 (1988)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.53
0.025
-
P1,P3-bis(5'-adenosyl) triphosphate
-
Euglena gracilis
2.7.7.53
0.027
-
P1,P4-bis(5'-adenosyl) tetraphosphate
-
Euglena gracilis
2.7.7.53
0.5
-
phosphate
-
Euglena gracilis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.53
divalent metal cations
requirement
Euglena gracilis
2.7.7.53
additional information
no divalent metal cation requirement for NDP-arsenolysis or NDP/phosphate-exchange reaction
Euglena gracilis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.53
30000
-
-
Euglena gracilis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.53
Acanthamoeba castellanii
-
-
-
2.7.7.53
Euglena gracilis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.7.53
625fold
Euglena gracilis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.53
NDP + arsenate
-
643513
Euglena gracilis
NMP + phosphate + ?
-
643513
Euglena gracilis
?
2.7.7.53
NDP + phosphate
-
643513
Euglena gracilis
phosphate + NDP
-
643513
Euglena gracilis
r
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
i.e. AP3A
643513
Euglena gracilis
ADP
-
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
i.e. AP3A
643513
Acanthamoeba castellanii
ADP
-
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + chromate
-
643513
Euglena gracilis
?
-
-
-
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + molybdate
-
643513
Euglena gracilis
?
-
-
-
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
i.e. Ap4A or AppppA, substrate specificity
643513
Euglena gracilis
ADP + ATP
-
643513
Euglena gracilis
-
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
i.e. Ap4A or AppppA, substrate specificity
643513
Acanthamoeba castellanii
ADP + ATP
-
643513
Acanthamoeba castellanii
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + vanadate
-
643513
Euglena gracilis
?
-
-
-
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate with CH2-bridge between P3 and P4 + phosphate
i.e. ApppCH2pA, methylene group substituting the oxygen
643513
Euglena gracilis
?
-
-
-
?
2.7.7.53
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate
i.e. GP4G
643513
Euglena gracilis
GDP + GTP
-
-
-
?
2.7.7.53
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
arsenate can replace phosphate
643513
Euglena gracilis
ADP + p4A
-
-
-
?
2.7.7.53
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
i.e. AP5A
643513
Euglena gracilis
ADP + p4A
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.53
8
-
-
Euglena gracilis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.53
0.025
-
P1,P3-bis(5'-adenosyl) triphosphate
-
Euglena gracilis
2.7.7.53
0.027
-
P1,P4-bis(5'-adenosyl) tetraphosphate
-
Euglena gracilis
2.7.7.53
0.5
-
phosphate
-
Euglena gracilis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.53
divalent metal cations
requirement
Euglena gracilis
2.7.7.53
additional information
no divalent metal cation requirement for NDP-arsenolysis or NDP/phosphate-exchange reaction
Euglena gracilis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.53
30000
-
-
Euglena gracilis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.7.53
625fold
Euglena gracilis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.53
NDP + arsenate
-
643513
Euglena gracilis
NMP + phosphate + ?
-
643513
Euglena gracilis
?
2.7.7.53
NDP + phosphate
-
643513
Euglena gracilis
phosphate + NDP
-
643513
Euglena gracilis
r
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
i.e. AP3A
643513
Euglena gracilis
ADP
-
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
i.e. AP3A
643513
Acanthamoeba castellanii
ADP
-
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + chromate
-
643513
Euglena gracilis
?
-
-
-
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + molybdate
-
643513
Euglena gracilis
?
-
-
-
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
i.e. Ap4A or AppppA, substrate specificity
643513
Euglena gracilis
ADP + ATP
-
643513
Euglena gracilis
-
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
i.e. Ap4A or AppppA, substrate specificity
643513
Acanthamoeba castellanii
ADP + ATP
-
643513
Acanthamoeba castellanii
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + vanadate
-
643513
Euglena gracilis
?
-
-
-
r
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate with CH2-bridge between P3 and P4 + phosphate
i.e. ApppCH2pA, methylene group substituting the oxygen
643513
Euglena gracilis
?
-
-
-
?
2.7.7.53
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate
i.e. GP4G
643513
Euglena gracilis
GDP + GTP
-
-
-
?
2.7.7.53
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
arsenate can replace phosphate
643513
Euglena gracilis
ADP + p4A
-
-
-
?
2.7.7.53
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
i.e. AP5A
643513
Euglena gracilis
ADP + p4A
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.53
8
-
-
Euglena gracilis