BRENDA - Enzyme Database

Immunoaffinity chromatography of diadenosine 5,5-P1,P4-tetraphosphate phosphorylase from Saccharomyces cerevisiae

Avila, D.M.; Kaushal, V.; Barnes, L.D.; Biotechnol. Appl. Biochem. 12, 276-283 (1990)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.7.7.53
most stable in Tris or phosphate buffered saline, 20 mM potassium phosphate, pH 6.8, NaCl-solution or 0.2 M glycine plus 0.1 mM EGTA, pH 2.7
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.53
Saccharomyces cerevisiae
-
-
-
2.7.7.53
Saccharomyces cerevisiae CGY 339
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.7.53
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.7.53
18.8
-
purified enzyme
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.53
P1,P2-bis(5'-adenosyl) diphosphate + phosphate
i.e. AP2A
643509
Saccharomyces cerevisiae
?
-
-
-
?
2.7.7.53
P1,P2-bis(5'-adenosyl) diphosphate + phosphate
i.e. AP2A
643509
Saccharomyces cerevisiae CGY 339
?
-
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
i.e. AP3A
643509
Saccharomyces cerevisiae
ADP
-
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
i.e. AP3A
643509
Saccharomyces cerevisiae CGY 339
ADP
-
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643509
Saccharomyces cerevisiae
ADP + ATP
-
643509
Saccharomyces cerevisiae
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643509
Saccharomyces cerevisiae CGY 339
ADP + ATP
-
643509
Saccharomyces cerevisiae CGY 339
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.7.53
37
-
assay at
Saccharomyces cerevisiae
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.7.53
most stable in Tris or phosphate buffered saline, 20 mM potassium phosphate, pH 6.8, NaCl-solution or 0.2 M glycine plus 0.1 mM EGTA, pH 2.7
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.7.53
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.7.53
18.8
-
purified enzyme
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.53
P1,P2-bis(5'-adenosyl) diphosphate + phosphate
i.e. AP2A
643509
Saccharomyces cerevisiae
?
-
-
-
?
2.7.7.53
P1,P2-bis(5'-adenosyl) diphosphate + phosphate
i.e. AP2A
643509
Saccharomyces cerevisiae CGY 339
?
-
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
i.e. AP3A
643509
Saccharomyces cerevisiae
ADP
-
-
-
?
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
i.e. AP3A
643509
Saccharomyces cerevisiae CGY 339
ADP
-
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643509
Saccharomyces cerevisiae
ADP + ATP
-
643509
Saccharomyces cerevisiae
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643509
Saccharomyces cerevisiae CGY 339
ADP + ATP
-
643509
Saccharomyces cerevisiae CGY 339
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.7.53
37
-
assay at
Saccharomyces cerevisiae