BRENDA - Enzyme Database

Phosphorolytic cleavage of diadenosine 5,5-P1,P4-tetraphosphate. Properties of homogeneous diadenosine 5,5-P1,P4-tetraphosphate alpha, beta-phosphorylase from Saccharomyces cerevisiae

Guranowski, A.; Blanquet, S.; J. Biol. Chem. 260, 3542-3547 (1985)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.7.7.53
glycerol, 10%, stabilizes
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.53
dithioerythritol
-
Saccharomyces cerevisiae
2.7.7.53
iodoacetate
-
Saccharomyces cerevisiae
2.7.7.53
N-ethylmaleimide
-
Saccharomyces cerevisiae
2.7.7.53
p-hydroxymercuribenzoate
-
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.53
0.06
-
P1,P4-bis(5'-adenosyl) tetraphosphate
pH 8.2, 37°C
Saccharomyces cerevisiae
2.7.7.53
1
-
phosphate
pH 8.2, 37°C
Saccharomyces cerevisiae
2.7.7.53
3
-
arsenate
pH 8.2, 37°C
Saccharomyces cerevisiae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.53
Ca2+
less effective than Mg2+; requirement, less effective than Mn2+
Saccharomyces cerevisiae
2.7.7.53
Cd2+
slight activation
Saccharomyces cerevisiae
2.7.7.53
Co2+
activation, less effective than Mn2+ or Ca2+ or Mg2+
Saccharomyces cerevisiae
2.7.7.53
divalent metal cations
requirement
Saccharomyces cerevisiae
2.7.7.53
Mg2+
required
Saccharomyces cerevisiae
2.7.7.53
Mn2+
required
Saccharomyces cerevisiae
2.7.7.53
additional information
no activation by Ni2+
Saccharomyces cerevisiae
2.7.7.53
Zn2+
no effect
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.53
40000
-
gel filtration
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.7.53
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.7.53
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.7.53
55
-
purified enzyme
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.53
additional information
no substrates are 3'-adenosyltetraphosphate, poly(A), 3',5'-cAMP, NADP+, P1-bis(5'-adenosyl) monophosphate Ap2A, thymidine 5'-monophosphate-p-nitrophenylester
643507
Saccharomyces cerevisiae
?
-
-
-
-
2.7.7.53
additional information
no activity with sulfate
643507
Saccharomyces cerevisiae
?
-
-
-
-
2.7.7.53
additional information
no substrates are ADP, ATP, NAD+
643507
Saccharomyces cerevisiae
?
-
-
-
-
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
no activity with
643507
Saccharomyces cerevisiae
ADP
-
-
-
-
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate
arsenolysis, at about 80% the rate of phosphorolysis
643507
Saccharomyces cerevisiae
?
-
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643507
Saccharomyces cerevisiae
ADP + ATP
-
643507
Saccharomyces cerevisiae
?
2.7.7.53
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate
arsenate can replace phosphate
643507
Saccharomyces cerevisiae
GDP + GTP
-
643507
Saccharomyces cerevisiae
?
2.7.7.53
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate
i.e. GP4G
643507
Saccharomyces cerevisiae
GDP + GTP
-
643507
Saccharomyces cerevisiae
?
2.7.7.53
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
arsenate can replace phosphate
643507
Saccharomyces cerevisiae
ADP + p4A
-
643507
Saccharomyces cerevisiae
?
2.7.7.53
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
i.e. AP5A
643507
Saccharomyces cerevisiae
ADP + p4A
-
643507
Saccharomyces cerevisiae
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.7.53
monomer
1 * 40000, SDS-PAGE
Saccharomyces cerevisiae
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.7.53
37
-
assay at
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.53
8
-
P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Saccharomyces cerevisiae
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.7.53
6.5
9
about half-maximal activity at pH 6.5 and pH 9.0, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Saccharomyces cerevisiae
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.7.53
glycerol, 10%, stabilizes
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.7.53
dithioerythritol
-
Saccharomyces cerevisiae
2.7.7.53
iodoacetate
-
Saccharomyces cerevisiae
2.7.7.53
N-ethylmaleimide
-
Saccharomyces cerevisiae
2.7.7.53
p-hydroxymercuribenzoate
-
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.7.53
0.06
-
P1,P4-bis(5'-adenosyl) tetraphosphate
pH 8.2, 37°C
Saccharomyces cerevisiae
2.7.7.53
1
-
phosphate
pH 8.2, 37°C
Saccharomyces cerevisiae
2.7.7.53
3
-
arsenate
pH 8.2, 37°C
Saccharomyces cerevisiae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.7.53
Ca2+
less effective than Mg2+; requirement, less effective than Mn2+
Saccharomyces cerevisiae
2.7.7.53
Cd2+
slight activation
Saccharomyces cerevisiae
2.7.7.53
Co2+
activation, less effective than Mn2+ or Ca2+ or Mg2+
Saccharomyces cerevisiae
2.7.7.53
divalent metal cations
requirement
Saccharomyces cerevisiae
2.7.7.53
Mg2+
required
Saccharomyces cerevisiae
2.7.7.53
Mn2+
required
Saccharomyces cerevisiae
2.7.7.53
additional information
no activation by Ni2+
Saccharomyces cerevisiae
2.7.7.53
Zn2+
no effect
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.7.53
40000
-
gel filtration
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.7.53
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.7.53
55
-
purified enzyme
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.7.53
additional information
no substrates are 3'-adenosyltetraphosphate, poly(A), 3',5'-cAMP, NADP+, P1-bis(5'-adenosyl) monophosphate Ap2A, thymidine 5'-monophosphate-p-nitrophenylester
643507
Saccharomyces cerevisiae
?
-
-
-
-
2.7.7.53
additional information
no activity with sulfate
643507
Saccharomyces cerevisiae
?
-
-
-
-
2.7.7.53
additional information
no substrates are ADP, ATP, NAD+
643507
Saccharomyces cerevisiae
?
-
-
-
-
2.7.7.53
P1,P3-bis(5'-adenosyl) triphosphate + phosphate
no activity with
643507
Saccharomyces cerevisiae
ADP
-
-
-
-
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + arsenate
arsenolysis, at about 80% the rate of phosphorolysis
643507
Saccharomyces cerevisiae
?
-
-
-
?
2.7.7.53
P1,P4-bis(5'-adenosyl) tetraphosphate + phosphate
-
643507
Saccharomyces cerevisiae
ADP + ATP
-
643507
Saccharomyces cerevisiae
?
2.7.7.53
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate
arsenate can replace phosphate
643507
Saccharomyces cerevisiae
GDP + GTP
-
643507
Saccharomyces cerevisiae
?
2.7.7.53
P1,P4-bis(5'-guanosyl) tetraphosphate + phosphate
i.e. GP4G
643507
Saccharomyces cerevisiae
GDP + GTP
-
643507
Saccharomyces cerevisiae
?
2.7.7.53
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
arsenate can replace phosphate
643507
Saccharomyces cerevisiae
ADP + p4A
-
643507
Saccharomyces cerevisiae
?
2.7.7.53
P1,P5-bis(5'-adenosyl) pentaphosphate + phosphate
i.e. AP5A
643507
Saccharomyces cerevisiae
ADP + p4A
-
643507
Saccharomyces cerevisiae
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.7.53
monomer
1 * 40000, SDS-PAGE
Saccharomyces cerevisiae
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.7.53
37
-
assay at
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.7.53
8
-
P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Saccharomyces cerevisiae
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.7.53
6.5
9
about half-maximal activity at pH 6.5 and pH 9.0, P1,P4-bis(5'-adenosyl) tetraphosphate-phosphorolysis
Saccharomyces cerevisiae