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Literature summary extracted from

  • Trippe, R.; Richly, H.; Benecke, B.J.
    Biochemical characterization of a U6 small nuclear RNA-specific terminal uridylyltransferase (2003), Eur. J. Biochem., 270, 971-980.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.52 Ionic strength stimulates, e.g. 600 mM KCl Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.52 Mg2+ dependent on Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.52 115000
-
 x * 115000, U6-TUTase, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.52 Homo sapiens
-
 an unspecific enzyme form and an enzyme form specific for U6 sn-RNA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.52 partial purification of U6 snRNA specific enzyme form via chromatography on a U6 snRNA affinity resin, separation from unspecific enzyme form Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.52 HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.52 UTP + RNAn specifically modifies the 3'-UMP terminal of mammalian U6 small nuclear RNA, i.e. snRNA, structural requirements and specificity, overview Homo sapiens diphosphate + RNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.52 ? x * 115000, U6-TUTase, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.7.52 U6-TUTase U6 snRNA-specific terminal uridylyltransferase Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.52 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.52 7.9
-
 assay at Homo sapiens