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Literature summary extracted from

  • Ernst, N.L.; Panicucci, B.; Igo, R.P., Jr.; Panigrahi, A.K.; Salavati, R.; Stuart, K.
    TbMP57 is a 3' terminal uridylyl transferase (TUTase) of the Trypanosoma brucei editosome (2003), Mol. Cell, 11, 1525-1536.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.52 protein TbMP81 editosome protein, interaction enhances the enzyme activity Trypanosoma brucei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.52 DNA sequence determination, editosomal enzyme, expression as His-tagged protein in Escherichia coli Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.52 mitochondrion
-
 Trypanosoma brucei 5739
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2.7.7.52 additional information integral editosome protein, distinct from mitochondrial enzyme form Trypanosoma brucei
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.52 500000
-
 mitochondrial enzyme form, gel filtration Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.52 UTP + RNAn Trypanosoma brucei addition of primarily single U to single-stranded RNA, addition of the number of Us specified by a guide RNA to insertion editing-like substrates diphosphate + RNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.52 Trypanosoma brucei
-
 editosome protein TbMP57, distinct from mitochondrial enzyme form, i.e. TbTUTase 108
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.52 recombinant His-tagged enzyme from Escherichia coli Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.52 ATP + RNAn editosomal enzyme form, no activity Trypanosoma brucei diphosphate + RNAn+1
-
 ?
2.7.7.52 CTP + RNAn editosomal enzyme form, no activity Trypanosoma brucei diphosphate + RNAn+1
-
 ?
2.7.7.52 GTP + RNAn editosomal enzyme form, no activity Trypanosoma brucei diphosphate + RNAn+1
-
 ?
2.7.7.52 UTP + RNAn editosomal enzyme form shows preference for a 3' terminal A or G, while the mitochondrial enzyme form does not Trypanosoma brucei diphosphate + RNAn+1
-
 ?
2.7.7.52 UTP + RNAn mitochondrial enzyme form adds Us at the 3' and the 5' end of the RNA Trypanosoma brucei diphosphate + RNAn+1
-
 ?
2.7.7.52 UTP + RNAn addition of primarily single U to single-stranded RNA, addition of the number of Us specified by a guide RNA to insertion editing-like substrates Trypanosoma brucei diphosphate + RNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.52 3' terminal uridylyl transferase
-
 Trypanosoma brucei