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Literature summary extracted from

  • Aphasizhev, R.; Sbicego, S.; Peris, M.; Jang, S.H.; Aphasizheva, I.; Simpson, A.M.; Rivlin, A.; Simpson, L.
    Trypanosome mitochondrial 3' terminal uridylyl transferase (TUTase): the key enzyme in U-insertion/deletion RNA editing (2002), Cell, 108, 637-648.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.52 DNA sequence determination, gene is located on chromosome 9, expression as His-tagged protein in Escherichia coli Leishmania tarentolae
2.7.7.52 DNA sequence determination, mitochondrial enzyme, expression as His-tagged protein in Escherichia coli Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.52 brRNA inhibits the number of added residues Leishmania tarentolae
2.7.7.52 additional information RNA interference, i.e. RNAi, induced by tetracyclines causes down-regulation of RET1 and RET2 expression in vitro and in vivo Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.52 mitochondrion
-
 Trypanosoma brucei 5739
-
2.7.7.52 mitochondrion
-
 Leishmania tarentolae 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.52 Mg2+ required Leishmania tarentolae
2.7.7.52 Mn2+ requires Mn2+ or Mg2+ Leishmania tarentolae
2.7.7.52 Mn2+ selectively inhibits the distributive activity of the enzyme Leishmania tarentolae
2.7.7.52 Mn2+ when GTP, CTP, or ATP are used as substrates, Mn2+ is more effective than Mg2+ Leishmania tarentolae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.52 135000
-
 native PAGE Leishmania tarentolae
2.7.7.52 500000
-
 native PAGE and gel filtration Trypanosoma brucei
2.7.7.52 700000
-
 RNA-stabilized higher complexed form, native PAGE and glycerol gradient sedimentation Trypanosoma brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.52 additional information Trypanosoma brucei
-
 ?
-
 ?
2.7.7.52 additional information Leishmania tarentolae enzyme interacts with a minor fraction of total RNA ligase ?
-
 ?
2.7.7.52 UTP + RNAn Trypanosoma brucei
-
 diphosphate + RNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.52 Leishmania tarentolae
-
-
-
2.7.7.52 Trypanosoma brucei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.52 recombinant His-tagged enzyme from Escherichia coli Trypanosoma brucei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.52 6.67
-
 purified enzyme Leishmania tarentolae
2.7.7.52 8.2
-
 purified recombinant enzyme Leishmania tarentolae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.52 ATP + RNAn low activity Leishmania tarentolae diphosphate + RNAn+1
-
 ?
2.7.7.52 CTP + RNAn
-
 Leishmania tarentolae diphosphate + RNAn+1
-
 ?
2.7.7.52 GTP + RNAn about 50% of the activity with UTP, prefers Mn2+ as divalent cation Leishmania tarentolae diphosphate + RNAn+1
-
 ?
2.7.7.52 additional information
-
 Trypanosoma brucei ?
-
 ?
2.7.7.52 additional information enzyme interacts with a minor fraction of total RNA ligase Leishmania tarentolae ?
-
 ?
2.7.7.52 UTP + RNAn
-
 Trypanosoma brucei diphosphate + RNAn+1
-
 ?
2.7.7.52 UTP + RNAn RNA substrate specificity, overview Leishmania tarentolae diphosphate + RNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.52 More enzyme is a member of the DNA polymerase beta nucleotidyltransferase superfamily Trypanosoma brucei
2.7.7.52 More enzyme is a member of the DNA polymerase beta nucleotidyltransferase superfamily Leishmania tarentolae